Course Name |
(中) 生醫組織工程學(6850) |
(Eng.) Biomedical Tissue Engineering |
Offering Dept |
Graduate Institute of Biomedical Engineering |
Course Type |
Elective |
Credits |
3 |
Teacher |
SHU-PING LIN |
Department |
Graduate Institute of Biomedical Engineering/Graduate |
Language |
English |
Semester |
2025-SPRING |
Course Description |
Tissue engineering is a multidisciplinary field incorporating the principles of biology, chemistry, engineering, and medicine to create biological substitutes of native tissues for scientific research or clinical use. Specific applications of this technology include studies of tissue development and function, investigating drug response, and tissue repair and replacement. This area is rapidly becoming one of the most promising treatment options for patients suffering from tissue failure. The course will cover the fundamental introduction of tissue engineering and cells, biomaterials and scaffolds in tissue engineering, in-vitro and in-vivo strategies, clinical applications of tissue engineering and ethical and regulatory issues in tissue engineering. |
Prerequisites |
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self-directed learning in the course |
N |
Relevance of Course Objectives and Core Learning Outcomes(%) |
Teaching and Assessment Methods for Course Objectives |
Course Objectives |
Competency Indicators |
Ratio(%) |
Teaching Methods |
Assessment Methods |
This course uses a combination of lectures and self-directed learning to the “tissue engineering”. 1. Combine the knowledge of tissue engineering with others advanced techniques in biomedical applications. 2. Understand the ethics and values in the design/development of engineered tissues. |
1.Possess professional knowledge in biomedical engineering |
2.Plan and implement research projects, and have the ability to solve problems independently. |
3.Ability to write scientific papers and communicate research results effectively. |
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Other |
topic Discussion/Production |
Discussion |
Lecturing |
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Written Presentation |
Attendance |
Oral Presentation |
Assignment |
Study Outcome |
Other |
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Course Content and Homework/Schedule/Tests Schedule |
Week |
Course Content |
Week 1 |
1.0 Introduction of Tissue Engineering
1.1 General Overview, Goals, and Applications of Tissue Engineering |
Week 2 |
2.0 Basic Principles of Cell Culture
2.1 Types of Cell Culture
2.2 Isolation of Cells for Culture
2.3 Subculture
2.4 Cryopreservation
2.5 Characterization and Validation |
Week 3 |
3.0 Mesenchymal Stem Cells for Tissue Engineering
3.1 Background and Logic
3.2 Preparation of Media and Reagents
3.3 Isolation of Human Marrow-Derived Mesenchymal Stem Cells (HMSCS)
3.4 Propagation of Mesenchymal Stem Cells
3.5 Cryopreservation and Thawing of HMSCS |
Week 4 |
4.0 Human Embryonic Stem Cell Culture for Tissue Engineering
4.1 Embryonic Stem Cells
4.2 Maintenance and Expansion of Human Embryonic Stem (HES) Cells
4.3 Induction of Differentiation in ES Cells
4.4 Isolation of Specific Cell Types from Cultures Originating from ES Cells
4.5 Scale-Up of ES Cells in Tissue Engineering
4.6 Protocols for Using ES Cells in Tissue Engineering |
Week 5 |
5.0 Tissue Engineering: Basic Considerations
5.1 Introduction
5.2 In vitro Cultivation of Engineered Tissues
5.3 Overview of Cell Sources
5.4 Media
5.5 Biomaterial Scaffolds
5.6 Cellular Photoencapsulation in Hydrogels
5.7 Bioreactors
5.8 Scaffold Seeding
5.9 Bioreactor Cultivation
5.10 Engineered Tissue Analysis |
Week 6 |
6.0 Cell Sources for Cartilage Tissue Engineering
6.1 Introduction
6.2 Preparation of Media and Reagents
6.3 Articular Chondrocytes for Cartilage Tissue Engineering
6.4 Cells with Chondrogenic Differentiation Potential
6.5 Chondrogenesis of Progenitor Cells in Vitro
6.6 Tissue Engineering Cartilage Implants from Cells with Chondrogenic Potential
6.7 Lipid-Mediated Gene Transfer for Cartilage Tissue Engineering |
Week 7 |
7.0 Tissue Engineering of Articular Cartilage
7.1 Culture Conditions for Chondrocytes
7.2 Harvesting Cartilage and Isolation of Chondrocytes
7.3 Culture of Chondrocytes and Fabrication of Cartilaginous Tissue by the Scaffold-Free
7.4 Criteria for Evaluation of Cartilaginous Tissue Formation |
Week 8 |
Midterm |
Week 9 |
8.0 Ligament Tissue Engineering
8.1 Tissue Harvest and Cell Isolation
8.2 Silk Matrices and RGD Surface Modification
8.3 Cell Seeding and Cultivation in Bioreactor
8.4 Analytical Assays |
Week 10 |
9.0 Tissue Engineering Human Skeletal Muscle for Clinical Applications
9.1 Introduction
9.2 Skeletal Muscle Needle Biopsy
9.3 Isolation and Culture of Human Skeletal Myoblasts |
Week 11 |
9.4 Characterization of Human Skeletal Myoblasts
9.5 Tissue Engineering of Human Skeletal Myoblasts with Clinically Approved Extracellular Matrix Materials
9.6 Applications of Tissue-Engineered Human Skeletal Muscle |
Week 12 |
10.0 Engineered Heart Tissue (EHT)
10.1 Background of EHT
10.2 Isolation and Culture Methodology
10.3 Gene Transfer in Engineered Heart Tissue
10.4 Histologic Procedures in Engineered Heart Tissue
10.5 Representative Tissue Culture Study |
Week 13 |
11.0 Tissue-Engineered Blood Vessels and Bones
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Week 14 |
12.0 Culture of Neuroendocrine and Neuronal Cells for Tissue Engineering
13.0 Tissue Engineering of the Liver |
Week 15 |
Final Exam |
Week 16 |
Final Presentation |
Week 17 |
Final Report |
Week 18 |
Final Report |
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Evaluation |
Attendance 5%, Midterm 35%, Final exam 35%, Oral Presentation 15% and 2-Page Final Report 10% |
Textbook & other References |
Principles of Tissue Engineering By: Robert Lanza, Robert Langer and Joseph Vacanti; Publisher: Academic Press; 4th edition (November 15, 2013)
Culture of Cells for Tissue Engineering (Culture of Specialized Cells) By: Gordana Vunjak-Novakovic and R. Ian Freshney; Publisher: Wiley-Liss; 1st edition (February 3, 2006). |
Teaching Aids & Teacher's Website |
iLearning
https://bioelecinterfacelab.wixsite.com/home |
Office Hours |
splin@dragon.nchu.edu.tw
1:00 pm~2:00 pm, Friday |
Sustainable Development Goals, SDGs |
03.Good Health and Well-Being   04.Quality Education   09.Industry, Innovation and Infrastructure | include experience courses:N |
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