NCHU Course Outline
Course Name (中) 生醫組織工程學(6849)
(Eng.) Biomedical Tissue Engineering
Offering Dept Graduate Institute of Biomedical Engineering
Course Type Elective Credits 3 Teacher Bill Cheng
Department Graduate Institute of Biomedical Engineering/Graduate Language English Semester 2024-FALL
Course Description Tissue engineering is a multidisciplinary field incorporating the principles of biology, chemistry, engineering, and medicine to create biological substitutes of native tissues for scientific research or clinical use. Specific applications of this technology include studies of tissue development and function, investigating drug response, and tissue repair and replacement. This area is rapidly becoming one of the most promising treatment options for patients suffering from tissue failure. The course will cover the fundamental introduction of tissue engineering and cells, biomaterials and scaffolds in tissue engineering, in-vitro and in-vivo strategies, clinical applications of tissue engineering and ethical and regulatory issues in tissue engineering.
Prerequisites
self-directed learning in the course N
Relevance of Course Objectives and Core Learning Outcomes(%) Teaching and Assessment Methods for Course Objectives
Course Objectives Competency Indicators Ratio(%) Teaching Methods Assessment Methods
This course uses a combination of lectures and self-directed learning to the “tissue engineering”. 1. Combine the knowledge of tissue engineering with others advanced techniques in biomedical applications. 2. Understand the ethics and values in the design/development of engineered tissues.
topic Discussion/Production
Discussion
Lecturing
Attendance
Oral Presentation
Course Content and Homework/Schedule/Tests Schedule
Week Course Content
Week 1 1.0 Introduction of Tissue Engineering
1.1 General Overview, Goals, and Applications of Tissue Engineering
Week 2 2.0 Basic Principles of Cell Culture
2.1 Types of Cell Culture
2.2 Isolation of Cells for Culture
2.3 Subculture
2.4 Cryopreservation
2.5 Characterization and Validation
Week 3 3.0 Mesenchymal Stem Cells for Tissue Engineering
3.1 Background and Logic
3.2 Preparation of Media and Reagents
3.3 Isolation of Human Marrow-Derived Mesenchymal Stem Cells (HMSCS)
3.4 Propagation of Mesenchymal Stem Cells
3.5 Cryopreservation and Thawing of HMSCS
Week 4 4.0 Human Embryonic Stem Cell Culture for Tissue Engineering
4.1 Embryonic Stem Cells
4.2 Maintenance and Expansion of Human Embryonic Stem (HES) Cells
4.3 Induction of Differentiation in ES Cells
4.4 Isolation of Specific Cell Types from Cultures Originating from ES Cells
4.5 Scale-Up of ES Cells in Tissue Engineering
4.6 Protocols for Using ES Cells in Tissue Engineering
Week 5 5.0 Tissue Engineering: Basic Considerations
5.1 Introduction
5.2 In vitro Cultivation of Engineered Tissues
5.3 Overview of Cell Sources
5.4 Media
5.5 Biomaterial Scaffolds
5.6 Cellular Photoencapsulation in Hydrogels
5.7 Bioreactors
5.8 Scaffold Seeding
5.9 Bioreactor Cultivation
5.10 Engineered Tissue Analysis
Week 6 6.0 Cell Sources for Cartilage Tissue Engineering
6.1 Introduction
6.2 Preparation of Media and Reagents
6.3 Articular Chondrocytes for Cartilage Tissue Engineering
6.4 Cells with Chondrogenic Differentiation Potential
6.5 Chondrogenesis of Progenitor Cells in Vitro
6.6 Tissue Engineering Cartilage Implants from Cells with Chondrogenic Potential
6.7 Lipid-Mediated Gene Transfer for Cartilage Tissue Engineering
Week 7 7.0 Tissue Engineering of Articular Cartilage
7.1 Culture Conditions for Chondrocytes
7.2 Harvesting Cartilage and Isolation of Chondrocytes
7.3 Culture of Chondrocytes and Fabrication of Cartilaginous Tissue by the Scaffold-Free
7.4 Criteria for Evaluation of Cartilaginous Tissue Formation
Week 8 Midterm
Week 9 8.0 Ligament Tissue Engineering
8.1 Tissue Harvest and Cell Isolation
8.2 Silk Matrices and RGD Surface Modification
8.3 Cell Seeding and Cultivation in Bioreactor
8.4 Analytical Assays
Week 10 9.0 Tissue Engineering Human Skeletal Muscle for Clinical Applications
9.1 Introduction
9.2 Skeletal Muscle Needle Biopsy
9.3 Isolation and Culture of Human Skeletal Myoblasts
Week 11 9.4 Characterization of Human Skeletal Myoblasts
9.5 Tissue Engineering of Human Skeletal Myoblasts with Clinically Approved Extracellular Matrix Materials
9.6 Applications of Tissue-Engineered Human Skeletal Muscle
Week 12 10.0 Engineered Heart Tissue (EHT)
10.1 Background of EHT
10.2 Isolation and Culture Methodology
10.3 Gene Transfer in Engineered Heart Tissue
10.4 Histologic Procedures in Engineered Heart Tissue
10.5 Representative Tissue Culture Study
Week 13 11.0 Tissue-Engineered Blood Vessels and Bones
11.1 Culture of Tissue-Engineered Vascular Grafts
11.2 Characterization of Engineered Vessels
Week 14 11.3 Isolation and Culture Methodology of hMSC for Tissue Engineering of Bone
11.4 Analytical and Histological Assays of Tissue-Engineered Bones
Week 15 12.0 Culture of Neuroendocrine and Neuronal Cells for Tissue Engineering
12.1 Three-Dimensional Neuroendocrine/Neuronal Differentiation of PC12 Pheochromocytoma Cells
12.2 Formation of Sertoli-NT2N Tissue Constructs to Treat Neurodegenerative Disease
12.3 The Dorsal Root Ganglion as a Model System
Week 16 13.0 Tissue Engineering of the Liver
13.1 Background of Liver Disease and Cell-Based Therapies
13.2 Isolation of Primary Rat Hepatocytes
13.3 Assays of Hepatocyte Function
13.4 Micropatterned Cell Cultures
13.5 Bioreactor System for the Regulation of Hepatocyte Zonal Heterogeneity
13.6 Photopatterned Three-Dimensional Hydrogels Containing Living Cells
Week 17 Final Exam
Week 18 Oral Presentation and Submission of Final Report
Evaluation
Attendance 5%, Midterm 35%, Final exam 35%, Oral Presentation 15% and 2-Page Final Report 10%
Textbook & other References

Teaching Aids & Teacher's Website

Office Hours
Friday 9-10am
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Update Date, year/month/day:None Printed Date, year/month/day:2024 / 10 / 04
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