NCHU Course Outline
Course Name (中) 植物基因轉殖技術(8012)
(Eng.) Transgenic Plant Technology
Offering Dept Graduate Institute of Biotechnology
Course Type Elective Credits 2 Teacher 孫德芬
Department Graduate Institute of BiotechnologyPh.D Language English Semester 2024-FALL
Course Description Learning theory and operation of plant transgenic technology
Prerequisites
self-directed learning in the course N
Relevance of Course Objectives and Core Learning Outcomes(%) Teaching and Assessment Methods for Course Objectives
Course Objectives Competency Indicators Ratio(%) Teaching Methods Assessment Methods
基因暨生化工程實驗室負責人的養成
1.Capable of Independently acquiring new knowledge about cutting-edge Biotechnology.
2.Capable of verbally expressing ideas and conveying opinions.
4.Capable of planning and designing laboratory experiments.
5.Capable of performing, testing, and manipulating laboratory experimental operations.
25
25
25
25
topic Discussion/Production
Exercises
Lecturing
Oral Presentation
Internship
Written Presentation
Course Content and Homework/Schedule/Tests Schedule
Week Course Content
Week 1 1-1. General introduction
2-1. Fluorescence & confocal microscopy
3-1. Agrobacteria transformation and growth
3-2. Stable transgenic technology-dipping
3-3. Stable transgenic plant-selection
4-1. Transgenic reporter gene-GUS staining & photography
4-2. Transgenic reporter gene-Luciferase activity assay
4-3. Transgenic reporter gene-GFP & fluorescence microscopy
5-1. Paper discussion I
6-1. Transient expression-protoplast isolation
6-2. Transient expression-PEG transformation
6-3. Confocal imaging-protoplast
7-1. Transient expression-particle bombardment
7-2. Confocal imaging-epidermal cell
8-1. Agarose section & confocal imaging
9-1. paper discussion II

Week 2
Week 3
Week 4
Week 5
Week 6
Week 7
Week 8
Week 9
Week 10
Week 11
Week 12
Week 13
Week 14
Week 15
Week 16
Week 17
Week 18
Evaluation
課堂討論(40%),實驗報告(60%)
Textbook & other References

Teaching Aids & Teacher's Website
教師自製教材
Office Hours
email or by appointment
Sustainable Development Goals, SDGs
 include experience courses:N
Please respect the intellectual property rights and use the materials legally.Please repsect gender equality.
Update Date, year/month/day:None Printed Date, year/month/day:2024 / 10 / 11
The second-hand book website:http://www.myub.com.tw/