NCHU Course Outline
Course Name (中) 現代光譜學-生物物理學與生物化學解析(6889)
(Eng.) Modern Optical Spectroscopy: With Exercises And Examples From Biophysi
Offering Dept Graduate Institute of Biomedical Engineering
Course Type Elective Credits 3 Teacher SHU-PING LIN
Department Graduate Institute of Biomedical Engineering/Graduate Language English Semester 2024-FALL
Course Description This course should give the knowledge of optical spectroscopy in solid-state molecules, cell biology, biophysics, and biochemistry. The course covers the introduction of optical instruments, atomic spectrum, spectra of proteins or nucleic acids, the photoelectric effect, fluorescence, IR and Raman spectroscopy, UV/VIS spectroscopy, Auger electron spectroscopy (AES), electron spectroscopy for chemical analysis (ESCA, also known as X-ray photoelectron spectroscopy (XPS)), Scanning Electron Microscopy (SEM) and EDS (energy-dispersive spectrometer) for the applications of chemical analysis, biochemistry, biophysics, and chemistry of surface material.
Prerequisites
self-directed learning in the course Y
Relevance of Course Objectives and Core Learning Outcomes(%) Teaching and Assessment Methods for Course Objectives
Course Objectives Competency Indicators Ratio(%) Teaching Methods Assessment Methods
The main objective of this course is how to apply the concepts of optical spectroscopy to investigate and characterize the molecules, cell biology, biophysics, and biochemistry.
1.Possess professional knowledge in biomedical engineering.
2.Plan and implement research projects, and have the ability to solve problems independently.
3.Ability to write scientific papers and communicate research results effectively.
4.Integration in interdisciplinary research and innovative research skills.
5.Possess insightful perspective on industry and globalization.
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20
20
10
10
Lecturing
Discussion
Exercises
topic Discussion/Production
Networking/Distance Education
Quiz
Assignment
Oral Presentation
Attendance
Written Presentation
Course Content and Homework/Schedule/Tests Schedule
Week Course Content
Week 1 1. Introduction
Week 2 2. The Beer-Lambert Law
Week 3 3. Absorption Spectra of Mixtures, Distortions of Absorption Spectra by Light Scattering or Nonuniform Distributions of the Absorbing Molecules
Week 4 4. UV/VIS spectra
Week 5 5. Vibrational Adsorption
Week 6 6. Absorption Spectra of Proteins and Nucleic Acids
Week 7 7. IR spectra
Week 8 8. Techniques for Measuring Absorbance
Week 9 9. Introduction of the Photoelectric Effect
Week 10 10.Lasers
Week 11 11.Raman spectra, ESCA spectra, and AES spectra
Week 12 12.Analysis of ESCA spectra by XPS peak software
Week 13 13.Fluorescence – (Fluorescence Yields and Lifetimes, Photobleaching, Fluorescent Probes and Tags)
Week 14 14.Single-Molecule, High-Resolution Microscopy, Scanning Electron Microscopy (SEM) and EDS (energy-dispersive spectrometer)
Week 15 15.Final Exam
Week 16 16.Project – Paper Reading and Discussion
Week 17 17.Project – Paper Reading and Discussion
Week 18 18.Final Report
Evaluation
Attendance 5%, Homework 15%, Midterm 25%, Final Exam 25%, Oral presentation 15%,Final Report 15%
Textbook & other References
1. Modern Optical Spectroscopy: With Exercises and Examples from Biophysics and Biochemistry, Springer, by William W. Parson, 2nd printing edition, 2009.2.
2. Photoelectron Spectroscopy: Principles and Applications, Springer Berlin Heidelberg, by Stephan Hüfner, 2010.
Teaching Aids & Teacher's Website
iLearning
Office Hours
splin@dragon.nchu.edu.tw
Wednesday, 1:00 pm-3:00 pm
Sustainable Development Goals, SDGs
04.Quality Educationinclude experience courses:N
Please respect the intellectual property rights and use the materials legally.Please repsect gender equality.
Update Date, year/month/day:2024/09/02 17:32:24 Printed Date, year/month/day:2024 / 10 / 04
The second-hand book website:http://www.myub.com.tw/