NCHU Course Outline
Course Name (中) 植物病原分子檢測技術(6213)
(Eng.) Molecular Detection Technology on Plant Pathogens
Offering Dept Department of Plant Pathology
Course Type Elective Credits 2 Teacher Li-Hung Chen
Department Master Program in Plant Medicine and Good Agricultural Practice/Graduate Language English Semester 2024-FALL
Course Description 本課程將應用分生相關技術(PCR、RT-qPCR、ELISA等)於植物病原的檢測與鑑定。
This class will teach students how to apply molecular techniques for plant disease diagnosis.
Prerequisites
self-directed learning in the course Y
Relevance of Course Objectives and Core Learning Outcomes(%) Teaching and Assessment Methods for Course Objectives
Course Objectives Competency Indicators Ratio(%) Teaching Methods Assessment Methods
學生們將於此實習課程中,學習與操作到分生相關實驗技術,也將會於課堂中討論各種新的分生技術與其在植物病理鑑定上的應用。
Students will study molecular techniques for plant disease diagnosis. We will discuss the new techniques in the class.
2.To develop the abilities of plant clinical diagnosis and prescribing pesticides
3.Techniques and knowledge of plant quarantine and disease management
5.Development of the ability to conduct scientific research, to work in a team and to present and interpret scientific results
40
40
20
Lecturing
Practicum
topic Discussion/Production
Internship
Oral Presentation
Course Content and Homework/Schedule/Tests Schedule
Week Course Content
Week 1 Introduction
Week 2 Collect disease sample/Isolate pathogen
Week 3 DNA extraction
Week 4 DNA extraction and ITS PCR
Week 5 Analyze ITS sequence and molecular cloning_Ligation
Week 6 Molecular cloning_E.coli Transformation
Week 7 Molecular cloning_Plasmid extraction
Week 8 RNA extraction
Week 9 RT-PCR
Week 10 design qPCR primers and microbial genome search
Week 11 qPCR /Submit lab notebook and the title of your report.
Week 12 LAMP PCR
Week 13 Microscope
Week 14 Protein extraction and SDS PAGE
Week 15 ELISA
Week 16 Immunostrip
Week 17 Group presentation
Week 18 Submit lab notebook
Evaluation
出席狀況(30%)、口頭報告(30%)、實習參與(40%)
Attendance (30%), Oral presentation (30%) and experiment (40%)
Textbook & other References

Teaching Aids & Teacher's Website
Power point presentation
Office Hours
Please directly contact the instructor.
Sustainable Development Goals, SDGs
02.Zero Hunger   03.Good Health and Well-Beinginclude experience courses:N
Please respect the intellectual property rights and use the materials legally.Please repsect gender equality.
Update Date, year/month/day:None Printed Date, year/month/day:2024 / 10 / 13
The second-hand book website:http://www.myub.com.tw/