NCHU Course Outline
Course Name (中) 蛋白質純化和X光晶體繞射學(6416)
(Eng.) Protein Purification and X-Ray Crystallography
Offering Dept Department of Food Science and Biotechnology
Course Type Elective Credits 2 Teacher Yeh Chen
Department Department of Food Science and Biotechnology/Graduate Language English Semester 2024-FALL
Course Description 本課程旨在教導學生學習並掌握如何正確地表達及純化重組蛋白、如何培養蛋白質晶體以及如何收集X光繞射數據並解析其結構,使學生能夠掌握此研究生命科學不可或缺的一大利器
Prerequisites
self-directed learning in the course Y
Relevance of Course Objectives and Core Learning Outcomes(%) Teaching and Assessment Methods for Course Objectives
Course Objectives Competency Indicators Ratio(%) Teaching Methods Assessment Methods
1.瞭解蛋白質結晶的基本原理To understand the basic principles of protein crystallography
2.瞭解如何收集並處理X光繞射數據To understand how to collect and process X-ray diffraction data
3.瞭解如何建立蛋白質的3D立體結構To understand how to build three-dimensional structure of protein
topic Discussion/Production
Discussion
Oral Presentation
Quiz
Course Content and Homework/Schedule/Tests Schedule
Week Course Content
Week 1 介紹重組蛋白質技術 Introduction of Recombinant Protein Production
Week 2 介紹不同的蛋白質表達系統_大腸桿菌及酵母菌系統 Introduction of E. coli and Yeast Expression Systems
Week 3 介紹不同的蛋白質表達系統_昆蟲細胞及哺乳細胞 Introduction of Insect Cell and Mammalian Cell Expression Systems
Week 4 融合蛋白技術 Fusion Protein Techniques
Week 5 蛋白純化原理介紹 Basic Principles of Protein Purification
Week 6 蛋白質純化技術與策略(一) Techniques and Strategies of Protein Purification
Week 7 蛋白質純化技術與策略(二) Techniques and Strategies of Protein Purification
Week 8 蛋白質純化技術與策略(三) Techniques and Strategies of Protein Purification
Week 9 期中測驗 Mid-Term Exam
Week 10 蛋白質晶體學概論 Introduction of Protein Crystallography
Week 11 晶體對稱性 Crystal Symmetry
Week 12 蛋白結晶原理 Protein Crystallization Experiments
Week 13 蛋白結晶實驗 Protein Crystallization Experiments
Week 14 X光繞射學 X-Ray Diffraction
Week 15 相位角解析技術_分子取代法 Phasing Methods_Molecular Replacement
Week 16 自主練習 autonomic learning
Week 17 自主練習 autonomic learning
Week 18 期末測驗
Evaluation
期中、期末筆試,出席狀況,課堂參與表現
Textbook & other References

Teaching Aids & Teacher's Website

Office Hours

Sustainable Development Goals, SDGs
07.Affordable and Clean Energy   14.Life Below Water   15.Life On Land   17.Partnerships for the Goalsinclude experience courses:N
Please respect the intellectual property rights and use the materials legally.Please repsect gender equality.
Update Date, year/month/day:2024/09/03 14:57:03 Printed Date, year/month/day:2024 / 10 / 11
The second-hand book website:http://www.myub.com.tw/