NCHU Course Outline
Course Name (中) 植物病原分子檢測技術(5212)
(Eng.) Molecular Detection Technology on Plant Pathogens
Offering Dept Department of Plant Pathology
Course Type Elective Credits 2 Teacher Li-Hung Chen
Department Department of Plant Pathology/Graduate Language English Semester 2026-FALL
Course Description 本課程將應用分生相關技術(PCR、RT-qPCR、ELISA等)於植物病原的檢測與鑑定。
Prerequisites
self-directed learning in the course Y
Relevance of Course Objectives and Core Learning Outcomes(%) Teaching and Assessment Methods for Course Objectives
Course Objectives Competency Indicators Ratio(%) Teaching Methods Assessment Methods
學生們將於此實習課程中,學習與操作到分生相關實驗技術,也將會於課堂中討論各種新的分生技術與其在植物病理鑑定上的應用。
3.Skills on agricultural biotechnology and its application
100
Course Content and Homework/Schedule/Tests Schedule
Week Course Content
Week 1 本課程一共分為三個部分

第一部分(1~6週)為
1. 樣本採集與保存
2. DNA的萃取
3. RNA的萃取
4. Protein的萃取

第二部分(7~14週)為
1. PCR
2. ITS檢測
3. RT-PCR
4. qPCR
5. lamp PCR

第三部分(15-18週)
6. 抗體與抗原
7. ELISA
8. immunostrip
Week 2
Week 3
Week 4
Week 5
Week 6
Week 7
Week 8
Week 9
Week 10
Week 11
Week 12
Week 13
Week 14
Week 15
Week 16
self-directed
learning

Evaluation
出席狀況(30%)、口頭報告(30%)、實習參與(40%)
Textbook & other References

Teaching Aids & Teacher's Website

Office Hours
週四上午10:00~12:00
Sustainable Development Goals, SDGs(Link URL)
 include experience courses:N
Please respect the intellectual property rights and use the materials legally.Please respect gender equality.
Update Date, year/month/day:None Printed Date, year/month/day:2026 / 10 / 17
The second-hand book website:http://www.myub.com.tw/