| Week |
Course Content |
| Week 1 |
Holiday |
| Week 2 |
Course Introduction：
Overview of course objectives, grading policy, and laboratory safety guidelines. |
| Week 3 |
Genomic DNA Extraction：
Principles and protocols for extracting high-quality DNA from plant tissues. |
| Week 4 |
DNA Quality Control (PCR & Gel Electrophoresis)：
Assessing DNA integrity and quantity using PCR amplification and agarose gel electrophoresis. |
| Week 5 |
ddRAD Library Construction I：
Digestion & Ligation: Restriction enzyme digestion of genomic DNA and adapter ligation for reduced representation sequencing. |
| Week 6 |
Holiday |
| Week 7 |
ddRAD Library Construction II：
Amplification & Purification: PCR amplification of the library fragments and final size selection/purification for sequencing. |
| Week 8 |
Introduction to Linux：
Installation of Linux environment (e.g., WSL) and essential command-line operations for bioinformatics. |
| Week 9 |
Introduction to R & RStudio：
Setting up the R environment; learning basic syntax, data structures, and package management. |
| Week 10 |
Holiday |
| Week 11 |
Statistical Analysis in R (ANOVA)：
Performing Analysis of Variance (ANOVA) to test hypotheses on biological datasets. |
| Week 12 |
Data Visualization in R：
transforming raw data into high-quality scientific figures |
| Week 13 |
Image Analysis I (Phenotyping)：
Introduction to digital image processing tools for identifying plant leaf traits. |
| Week 14 |
Image Analysis II (Phenotyping)：
Advanced quantification of phenotypic traits and data export for downstream analysis. |
| Week 15 |
Literature Review Presentation I：
Individual presentations on recent advancements in agricultural genomics and biotechnology. |
| Week 16 |
Literature Review Presentation II：
Individual presentations on recent advancements in agricultural genomics and biotechnology. |
self-directed
learning |
01.Participation in professional forums, lectures, and corporate sharing sessions related to industry-government-academia-research exchange activities.
02.Viewing multimedia materials related to industry and academia.
03.Preparing presentations or reports related to industry and academia.
05.Participation in various workshops organized by different departments of NCHU.
|