NCHU Course Outline

Course Name	(中) 作物分子育種學(6154)
Course Name	(Eng.) Crop Molecular Breeding
Offering Dept	Department of Agronomy
Course Type	Elective	Credits	3	Teacher	WANG, CHANG-SHENG
Department	Master Program in Specialty Crops and Metabolomics/Graduate	Language	中/英文	Semester	2024-FALL
Course Description	介紹作物育種家所需之基本遺傳學、分子遺傳技術、分子標誌與性狀關係、利用分子標誌輔助選拔、標定與選殖性狀基因之原理、轉殖植株所需之理論與實務，藉由原理解說、實例與實驗觀摩、操作，使同學們了解如何利用分子生物技術與分子遺傳理論，進行作物之分子育種。
Prerequisites				self-directed learning in the course	N

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本課程的目的是讓學生能學以致用，自己真的學會操作課程所傳授的技術。所以課程安排以知難行易的基礎進行，由操作中學習，邊做邊學，配合試驗操作原理，兼顧理論與實務，以達到知行合一的境界。為維持授課與學習品質，因此本課程以12人為限。講義是英文為主歡迎外籍生選課。

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Week	Course Content
Week 1	Review on the traditional breeding and methods
Week 2	Genetics required for practice plant breeding
Week 3	The central dogma of genetics Lab section 1. Sampling and treatments and genomic DNA extraction: mini- and HMW- DNA
Week 4	The central dogma of plant breeding
Week 5	Germplasm characterization and application in plant improvement in the 21 century
Week 6	Molecular markers and genetics Lab section 2. PCR based marker screening: Agarose gel and PAGE – RAPD, SSR, SCAR, CAPS
Week 7	Methods for Mapping genes
Week 8	Development and validation of molecular markers
Week 9	Breeding by design and smart crossing Lab section 3. Phenotype and genotype integration in R/QTL and QTL mapping
Week 10	Prediction and selection of plant breeding
Week 11	Marker-assisted selection and gene pyramiding
Week 12	Genomic breeding and genome wide selection Lab section 4. Marker development and design from linkage mapping
Week 13	Plant breeding with assistance of software
Week 14	Successful examples of plant breeding through molecular approach
Week 15	Marker assisted selection and genomic selection Lab section 5. Test of functional markers
Week 16	New plant breeding techniques
Week 17	Examples of crop breeding
Week 18	Future prospects of plant breeding Lab section 6. Gene pyramiding in MAS

Evaluation

I.平時考 30%、期末考 30%、期末報告 30%及指定作業 10%。
II.期末報告須知:
1.文獻報告。
2.繳交期限:報告前一周交 PPT。

Textbook & other References

I.平時考 30%、期末考 30%、期末報告 30%及指定作業 10%。
II.期末報告須知:
1.文獻報告。
2.繳交期限:報告前一周交 PPT。

1.Principles of plant genetics and breeding. 2012. Geroge Acquaah. Wiley-Blackwell.
2.Molecular Plant Breeding. 2010. Yunbi Xu. CABI.
3.Biotechnology-and-Plant-Breeding 2014.

參考文獻
1.Bernardo R. 2010. Genome-wide selection with minimal crossing in self-pollinated crops. Crop Sci. 50: 624-627.
2.Chang, TS, C-S Wang and Chien-Chen Lai et. al. (2017, Nov). Mapping and comparative proteomic analysis of the starch biosynthetic pathway in rice by 2D PAGE/MS. Plant Molecular Biology, 95(4), 333-343. (SCI).
3.Chen, R. K., M. H. Tsai, and K. Y. Chen. 2013. The construction of a random-type SNP molecular marker database for Taiwanese japonica rice varieties. Res Bull Tainan Dist Agric Improv Stn. 61:15-28.
4.Chung, Y. S., and Kim, C. S. et. al. 2017. Genotyping-by-sequencing: a promising tool for plant genetics research and breeding. Hort Environ Biotechnol. 58:425-431.
5.Cobb, J. N., and J. D. Platten et al. 2019. Back to the future: revisiting MAS as a tool for modern plant breeding. Theor Appl Genet. 132:647.
6.Collard, B. C, Y., and D. J. Mackill. 2008. Marker-assisted selection: an approach for precision plant breeding in the twenty-first century. Phil Trans R Soc. B. 363:557-572.
7.Elshire, R. J., and, Mitchell, S. E., et al. 2011. A robust, simple genotyping-by-sequencing (GBS) approach for high-diversity species. PLOS ONE. 6:e19379.
8.Fadiji AE, Santoyo G, Yadav AN and Babalola OO (2022) Efforts towards overcoming drought stress in crops: Revisiting the mechanisms employed by plant growth-promoting bacteria. Front. Microbiol. 13:962427.
9.Godwin,I.D., J. and I.T.Hickey., et al. 2019. Technological perspectives for plant breeding. Theor Appl Genet,132:555-557.
10.Ghazy, M.I., and Sallam, A. et. al. Utilization of genetic diversity and marker-trait to improve drought tolerance in rice (Oryza sativa L.). Mol Biol Rep 48, 157–170 (2021).
11.Huang, M., and C. H. Sneller., et. al. 2019. Use of genomic selection in breeding rice (Oryza sativa L.) for resistance to rice blast (Magnaporthe oryzae). Molecular Breed. 39:114.
12.Lo, K. L and C S Wang et. al. 2022. Two genomic regions of a sodium azide-induced rice mutant confer broad-spectrum and durable resistance to blast disease. Rice. 15:2. (SCI).
13.Meuwissen, T. H. E, and M. E. Goddard. Et. al. 2001. Prediction of total genetic value using genome-wide dense marker maps. Genetics. 157:1819-1829.
14.Mir, RR, and RK Varshney., et al. 2012 Integrated genomics, physiology and breeding approaches for improving drought tolerance in crops. Theoretical and Applied Genetics 125, 625-645.
15.Morris, G. P., S. P. Deshpande, and et. al. 2013. Population genomic and genome-wide association studies of agroclimatic traits in sorghum. Proc Natl Acad Sci USA.110:453-458.
16.Muthu V, and, Kambale R, et al. (2020) Pyramiding QTLs controlling tolerance against drought, salinity, and submergence in rice through marker-assisted breeding. PLoS ONE 15(1): e0227421.https://doi.org/10.1371/journal.pone.0227421
17.Nakaya, A., and S. N. Isobe. 2012. Will genomic selection be a practical method for plant breeding? Ann Bot. 110:1303-1316.
18.Newell, M. A., and J. L. Jannink. 2014. Genomic selection in plant breeding. Methods Mol Biol. 1145:117-130.
19.Poland, J. A., and T. W. Rife. 2012. Genotyping- by-sequencing for plant breeding and genetics. Plant Genome. 5:92-102.
20.Rasheed, A., Y. and Z. He. Et al. 2017. Crop breeding chips and genotyping platforms: progress, challenges, and perspectives. Mol Plant. 10:1047-1064.
21.Ribaut, J. M., and X. Delannay., et. al. 2010. Molecular breeding in developing countries: challenges and perspectives. Curr Opin Plant Biol. 13: 213-218.
22.Soto-Cerda, B. J., and S. Cloutier. 2012. Association mapping in plant genomes. InTech. DOI: 10.5772/33005.
23.Wang, C. S. and Lin, D. G. 2017. The application of genomic approaches in studying a bacterialblight-resistant mutant in rice. Advances in International Rice Research (ISBN:978-953-51-3010-9). InTech. DOI: 10.5772/67331.
24.Wang, C S et al. 2019. Sodium azide mutagenesis generated diverse and broad-spectrum blast resistance mutants in rice. Euphytica 215:145-156.
25.Xiong, W. and Y. Xu, et. al. Climate change challenges plant breeding. Current Opinion in Plant Biology 2022, 70: 102308.
26.Zingaretti, S. M. et al. 2013 Water Stress and Agriculture.http://dx.doi.org/10.5772/53877.
27.王強生 2021. 全基因體分析與先設計再育種—基因體育種是後基因體時代糧食作物的育種趨勢。前瞻基因體學技術於農業領域之研發應用與展望(ISBN:978-986-5452-17-9)(PP.7-26)。
28.王強生 2023.『因應氣候變遷之作物育種』. 2023 112年度農業生態系長期生態研究研討會,112年7月7日台南區農業改良場。

Teaching Aids & Teacher's Website

上傳iLearning 3.0 中英文皆有。

Office Hours

每周五下午2~5點須事先預約。

Sustainable Development Goals, SDGs

include experience courses：N

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Update Date, year/month/day：None	Printed Date, year/month/day：2024 / 9 / 08
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