| Course Name |
(中) 天然物生物活性篩選技術與實作(6853) |
| (Eng.) Techniques and Practice in Bioactivity Screening of Natural Products |
| Offering Dept |
Master Program in Specialty Crops and Metabolomics |
| Course Type |
Elective |
Credits |
3 |
Teacher |
YEN, PEI-LING |
| Department |
Master Program in Specialty Crops and Metabolomics/Graduate |
Language |
中/英文 |
Semester |
2026-FALL |
| Course Description |
This course provides students with practical and theoretical training in advanced functional screening strategies. Through lectures, laboratory practices, and project-based experiments, students will learn how to design, execute, and analyze screening assays to evaluate the biological activity of natural products. Emphasis is placed on lifespan, reproduction, locomotion, stress resistance, and behavior. Students will gain experience in assay optimization, troubleshooting, imaging, data visualization, and scientific presentation. The course culminates in a group project where students conduct a full functional screening study and present their findings. |
| Prerequisites |
|
self-directed learning in the course |
Y |
| Relevance of Course Objectives and Core Learning Outcomes(%) |
Teaching and Assessment Methods for Course Objectives |
| Course Objectives |
Competency Indicators |
Ratio(%) |
Teaching Methods |
Assessment Methods |
1. To cultivate students’ understanding of the fundamental principles and practical techniques of bioactivity screening of natural products.
2. To develop students’ hands-on skills in bioactivity screening using cellular and model organism systems. interpretation.
3. To enhance students’ ability in bioactivity data analysis and quality control. |
|
|
|
| Written Presentation |
| Attendance |
| Oral Presentation |
| Assignment |
|
| Course Content and Homework/Schedule/Tests Schedule |
| Week |
Course Content |
| Week 1 |
Introduction to Screening Concepts |
| Week 2 |
Experimental Design: Controls, Replicates, Statistical Power |
| Week 3 |
Lab: Synchronization and Setup |
| Week 4 |
Phenotypic Assays Overview |
| Week 5 |
Lab: Reproductive and Developmental Assays |
| Week 6 |
Lab: Stress Resistance Assay |
| Week 7 |
Lab: Locomotion Assay |
| Week 8 |
Midterm Presentation: Functional Screening Proposal |
| Week 9 |
Project Launch: Sample Selection & Screening Workflow Planning |
| Week 10 |
Project Lab 1: Setup of Assay (based on group topic) |
| Week 11 |
Progress Presentation I: Early Observations & Problem-solving Workshop |
| Week 12 |
Project Lab 2: Behavior (based on group topic) |
| Week 13 |
Progress Presentation II: Preliminary Results & Problem-solving Workshop |
| Week 14 |
Project Lab 3: Behavior (based on group topic) |
| Week 15 |
Data Processing and Visualization |
| Week 16 |
Final Project Presentations |
self-directed
learning |
03.Preparing presentations or reports related to industry and academia.
After completing the experiments, students are required to independently organize and analyze their experimental data, and to interpret the screening results through figures and concise reports, highlighting the biological significance and potential mechanisms of the observed bioactivities. Through this structured, stepwise approach to self-directed learning, students become familiar with bioactivity screening techniques for natural products and develop the ability to translate experimental findings into coherent scientific narratives. |
|
| Evaluation |
Attendance: 10%
Midterm assessment (exam or experimental assignments): 30%
Progress presentation (experimental progress and preliminary results): 30%
Final written report: 30% |
| Textbook & other References |
Meneely, P. M., Dahlberg, C. L., & Rose, J. K. (2019). Working with Worms: Caenorhabditis elegans as a Model Organism. Current Protocols Essential Laboratory Techniques, 19, e35. doi: 10.1002/cpet.35
Strange, Kevin, and SpringerLink. C. elegans Methods and Applications / Edited by Kevin Strange. Totowa, NJ: Humana Press, 2006. Web. |
| Teaching Aids & Teacher's Website |
| Lecture slides and selected research articles. |
| Office Hours |
| After class |
| Sustainable Development Goals, SDGs(Link URL) |
| include experience courses：N |
|