| Week |
Course Content |
| Week 1 |
1.0 Introduction of Tissue Engineering
1.1 General Overview, Goals, and Applications of Tissue Engineering |
| Week 2 |
2.0 Basic Principles of Cell Culture
2.1 Types of Cell Culture
2.2 Isolation of Cells for Culture
2.3 Subculture
2.4 Cryopreservation
2.5 Characterization and Validation |
| Week 3 |
3.0 Mesenchymal Stem Cells for Tissue Engineering
3.1 Background and Logic
3.2 Preparation of Media and Reagents
3.3 Isolation of Human Marrow-Derived Mesenchymal Stem Cells (HMSCS)
3.4 Propagation of Mesenchymal Stem Cells
3.5 Cryopreservation and Thawing of HMSCS |
| Week 4 |
4.0 Human Embryonic Stem Cell Culture for Tissue Engineering
4.1 Embryonic Stem Cells
4.2 Maintenance and Expansion of Human Embryonic Stem (HES) Cells
4.3 Induction of Differentiation in ES Cells
4.4 Isolation of Specific Cell Types from Cultures Originating from ES Cells
4.5 Scale-Up of ES Cells in Tissue Engineering
4.6 Protocols for Using ES Cells in Tissue Engineering |
| Week 5 |
5.0 Tissue Engineering: Basic Considerations
5.1 Introduction
5.2 In vitro Cultivation of Engineered Tissues
5.3 Overview of Cell Sources
5.4 Media
5.5 Biomaterial Scaffolds
5.6 Cellular Photoencapsulation in Hydrogels
5.7 Bioreactors
5.8 Scaffold Seeding
5.9 Bioreactor Cultivation
5.10 Engineered Tissue Analysis |
| Week 6 |
6.0 Cell Sources for Cartilage Tissue Engineering
6.1 Introduction
6.2 Preparation of Media and Reagents
6.3 Articular Chondrocytes for Cartilage Tissue Engineering
6.4 Cells with Chondrogenic Differentiation Potential
6.5 Chondrogenesis of Progenitor Cells in Vitro
6.6 Tissue Engineering Cartilage Implants from Cells with Chondrogenic Potential
6.7 Lipid-Mediated Gene Transfer for Cartilage Tissue Engineering |
| Week 7 |
7.0 Tissue Engineering of Articular Cartilage
7.1 Culture Conditions for Chondrocytes
7.2 Harvesting Cartilage and Isolation of Chondrocytes
7.3 Culture of Chondrocytes and Fabrication of Cartilaginous Tissue by the Scaffold-Free
7.4 Criteria for Evaluation of Cartilaginous Tissue Formation |
| Week 8 |
Midterm |
| Week 9 |
8.0 Ligament Tissue Engineering
8.1 Tissue Harvest and Cell Isolation
8.2 Silk Matrices and RGD Surface Modification
8.3 Cell Seeding and Cultivation in Bioreactor
8.4 Analytical Assays |
| Week 10 |
9.0 Tissue Engineering Human Skeletal Muscle for Clinical Applications
9.1 Introduction
9.2 Skeletal Muscle Needle Biopsy
9.3 Isolation and Culture of Human Skeletal Myoblasts |
| Week 11 |
9.4 Characterization of Human Skeletal Myoblasts
9.5 Tissue Engineering of Human Skeletal Myoblasts with Clinically Approved Extracellular Matrix Materials
9.6 Applications of Tissue-Engineered Human Skeletal Muscle |
| Week 12 |
10.0 Engineered Heart Tissue (EHT)
10.1 Background of EHT
10.2 Isolation and Culture Methodology
10.3 Gene Transfer in Engineered Heart Tissue
10.4 Histologic Procedures in Engineered Heart Tissue
10.5 Representative Tissue Culture Study |
| Week 13 |
11.0 Tissue-Engineered Blood Vessels and Bones
11.1 Culture of Tissue-Engineered Vascular Grafts
11.2 Characterization of Engineered Vessels |
| Week 14 |
11.3 Isolation and Culture Methodology of hMSC for Tissue Engineering of Bone
11.4 Analytical and Histological Assays of Tissue-Engineered Bones |
| Week 15 |
12.0 Culture of Neuroendocrine and Neuronal Cells for Tissue Engineering
12.1 Three-Dimensional Neuroendocrine/Neuronal Differentiation of PC12 Pheochromocytoma Cells
12.2 Formation of Sertoli-NT2N Tissue Constructs to Treat Neurodegenerative Disease
12.3 The Dorsal Root Ganglion as a Model System |
| Week 16 |
13.0 Tissue Engineering of the Liver
13.1 Background of Liver Disease and Cell-Based Therapies
13.2 Isolation of Primary Rat Hepatocytes
13.3 Assays of Hepatocyte Function
13.4 Micropatterned Cell Cultures
13.5 Bioreactor System for the Regulation of Hepatocyte Zonal Heterogeneity
13.6 Photopatterned Three-Dimensional Hydrogels Containing Living Cells |
| Week 17 |
Final Exam |
| Week 18 |
Oral Presentation and Submission of Final Report |