NCHU Course Outline
Course Name (中) 作物遺傳學特論(8062)
(Eng.) Advanced Crop Genetics
Offering Dept Department of Agronomy
Course Type Elective Credits 3 Teacher KU,HSIN-MEI
Department Department of AgronomyPh.D Language English Semester 2026-SPRING
Course Description This course is recommended for students who have previously taken Genetics, Plant Biotechnology, and Plant Virology. The content introduces research related to VIGE, exploring its theoretical strategies and applications. The teaching approach emphasizes presenting theories and the principles, logic, strategies, results, and inferences of related experiments. It focuses on training students in independent thinking and enhancing their ability to read and analyze relevant literature. In class, students are encouraged to ask questions and engage in interactive, two-way communication.
Prerequisites
self-directed learning in the course Y
Relevance of Course Objectives and Core Learning Outcomes(%) Teaching and Assessment Methods for Course Objectives
Course Objectives Competency Indicators Ratio(%) Teaching Methods Assessment Methods
1. Cognitive: Guide students to understand and identify the principles of VIGE.
2. Skills: Help students improve their ability to grasp and comprehend the purpose, methods, graphical results, and conclusions of each experiment when reading related literature.
1.
2.
25
75
Discussion
Lecturing
Written Presentation
Oral Presentation
Course Content and Homework/Schedule/Tests Schedule
Week Course Content
Week 1 1.Spök A, Sprink T, Allan AC, Yamaguchi T and Dayé C (2022), Towards social acceptability of genome-edited plants in industrialised countries? Emerging evidence from Europe, United States, Canada, Australia, New Zealand, and Japan. Front. Genome Ed. 4:899331. doi: 10.3389/fgeed.2022.899331
Week 2 2.Uranga M and José-Antonio Daròs 2022 Tools and targets: The dual role of plant viruses in CRISPR–Cas genome editing. Plant Genome. 2022;e20220
Week 3 3.Son S and Park SR (2022) Challenges Facing CRISPR/Cas9- Based Genome Editing in Plants. Front. Plant Sci. 13:902413. doi: 10.3389/fpls.2022.902413
Week 4 4.Ali Z et al 2015a Efficient virus-mediated genome editing in plants using the CRISPR/Cas9 system. Molecular Plant, 8, 1288–1291.
Week 5 5.Ali Z et al 2015b Activity and specificity of TRV mediated gene editing in plants. Plant Signal Behav. 10 (10), e1044191
Week 6 6.Ali Z et al 2018 Pea early-browning virus-mediated genome editing via the CRISPR/Cas9 system in Nicotiana benthamiana and Arabidopsis. Virus Research, 244, 333–337.
Week 7 244, 333–337.
7.Chen 2022 Development and optimization of a Barley stripe mosaic virus-mediated gene editing system to improve Fusarium head blight resistance in wheat. Plant Biotechnol J 1-3
Week 8 8.Midterm exame

Week 9 9.Li 2021 Highly efficient heritable genome editing in wheat using an RNA virus and bypassing tissue culture. Molecular Plant 14, 1787–1798
Week 10 10.Ma X et al 2020 Highly Efficient DNA-free Plant Genome Editing Using Virally Delivered CRISPR-Cas9. Nat. Plants 6, 773–779
Week 11 11.Ariga H et al 2020 Potato virus X vector-mediated DNA-free genome editing in plants. Plant Cell Physiol. 61, 1946-1953
Week 12 12.Zhang X et al 2020 An RNAi suppressor activates in planta virus–mediated gene editing. Functional & Integrative Genomics 20:471–477
Week 13 13.Chiong et al 2021 RNA silencing suppressor influenced performance of a virus vector delivering both guide RNA and Cas9 for CRISPR gene editing. Sci Report 11:6769
Week 14 14.Chaudhuri 2022 Classifcation of CRISPR/Cas system and its application in tomato breeding. Theoretical and Applied Genetics 135:367–387.
Week 15 15.Paul 2022 GE CROPSR: an automated platform for complex genome wide CRISPR gRNA design and validation. BMC Bioinformatics 23:74
Week 16 6.Usiak B 2016. Engineering synthetic gene circuits in living cells with CRISPR technology. Trends Biotechnol. 34:535–47Ϲ.Li T, Yang X, Yu Y, Si X, Zhai X, et al. 2018. Domestication of wild tomato is accelerated by genome editing. Nat. Biotechnol. 36:1160–63
final exame
self-directed
learning
   02.Viewing multimedia materials related to industry and academia.

Evaluation
口頭及書面報告
Textbook & other References
課堂文獻
Teaching Aids & Teacher's Website

Office Hours
周三4-5pm
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include experience courses:N
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Update Date, year/month/day:2026/01/01 14:13:21 Printed Date, year/month/day:2026 / 1 / 09
The second-hand book website:http://www.myub.com.tw/