| Course Name |
(中) 膜蛋白功能與結構(7315) |
| (Eng.) Functional and Structural Studies of Membrane Proteins |
| Offering Dept |
Graduate Institute of Biochemistry |
| Course Type |
Elective |
Credits |
3 |
Teacher |
HU NIEN JEN |
| Department |
Graduate Institute of Biochemistry/Graduate |
Language |
English |
Semester |
2026-SPRING |
| Course Description |
The cell membrane forms a hydrophobic barrier on the cell surface to maintain a constant concentration of biochemical substances within the cytoplasm. However, when a cell communicates with its environment or exchanges substances, it requires membrane proteins to assist water-soluble small molecules in penetrating this barrier. Consequently, membrane proteins play crucial roles in cellular physiology, nutrient exchange, signal transduction, and the immune system.
Human genome sequencing predicts that 30% of genes are transcribed into membrane proteins. For a long time, membrane proteins have been regarded as vital drug targets by pharmaceutical companies; currently, 50% of commercially available drugs are designed and developed to target membrane proteins. Therefore, understanding the function and structure of membrane proteins is essential for comprehending many fundamental questions in cellular physiology. This course will provide an in-depth exploration of the physiological functions, structural classifications, and biochemical instrumentation used for membrane proteins, as well as their future applications in pharmacology and clinical practice. |
| Prerequisites |
|
self-directed learning in the course |
Y |
| Relevance of Course Objectives and Core Learning Outcomes(%) |
Teaching and Assessment Methods for Course Objectives |
| Course Objectives |
Competency Indicators |
Ratio(%) |
Teaching Methods |
Assessment Methods |
| To enable students to understand the functions of membrane proteins from the perspective of their molecular 3D structures, and to apply this information to drug development and clinical diagnostics. |
|
|
| topic Discussion/Production |
| Discussion |
| Lecturing |
|
|
| Course Content and Homework/Schedule/Tests Schedule |
| Week |
Course Content |
| Week 1 |
Classification of membrane proteins
|
| Week 2 |
Membrane protein folding and insertion
|
| Week 3 |
Photoreaction center
|
| Week 4 |
Electron transport complex
|
| Week 5 |
Channels
|
| Week 6 |
Transporters
|
| Week 7 |
Receptors, G protein couple receptor (GPCR)
|
| Week 8 |
Mid-term exam |
| Week 9 |
Porin |
| Week 10 |
Membrane enzymes |
| Week 11 |
Pore-forming toxins |
| Week 12 |
Expression and purification of integral membrane protein |
| Week 13 |
Crystallization of integral membrane proteins
|
| Week 14 |
Functional studies of membrane proteins |
| Week 15 |
Structural studies of membrane proteins, Pharmaceutical applications |
| Week 16 |
Final oral presentation |
self-directed
learning |
03.Preparing presentations or reports related to industry and academia.
|
|
| Evaluation |
每週quiz問答
期中個人報告
期末小組報告 |
| Textbook & other References |
Biophysical Analysis of Membrane Proteins: Investigating Structure and Function, Eva Pebay-Peyroula
Membrane Structural Biology: With Biochemical and Biophysical Foundations, Mary Luckey
Journal publications in Nature, Science, Nature Structural & Molecular Biology, EMBO, PNAS, Nature Protocol, Nature Methods, etc.
|
| Teaching Aids & Teacher's Website |
Biophysical Analysis of Membrane Proteins: Investigating Structure and Function, Eva Pebay-Peyroula
Membrane Structural Biology: With Biochemical and Biophysical Foundations, Mary Luckey
Journal publications in Nature, Science, Nature Structural & Molecular Biology, EMBO, PNAS, Nature Protocol, Nature Methods, etc. |
| Office Hours |
| Every Tuesday 9:00-12:00 AM. |
| Sustainable Development Goals, SDGs(Link URL) |
| 04.Quality Education | include experience courses：N |
|