| Course Name |
(中) 現代光譜學－生物物理學與生物化學解析(6889) |
| (Eng.) Modern Optical Spectroscopy: With Exercises And Examples From Biophysi |
| Offering Dept |
Graduate Institute of Biomedical Engineering |
| Course Type |
Elective |
Credits |
3 |
Teacher |
SHU-PING LIN |
| Department |
Graduate Institute of Biomedical Engineering/Graduate |
Language |
English |
Semester |
2024-FALL |
| Course Description |
This course should give the knowledge of optical spectroscopy in solid-state molecules, cell biology, biophysics, and biochemistry. The course covers the introduction of optical instruments, atomic spectrum, spectra of proteins or nucleic acids, the photoelectric effect, fluorescence, IR and Raman spectroscopy, UV/VIS spectroscopy, Auger electron spectroscopy (AES), electron spectroscopy for chemical analysis (ESCA, also known as X-ray photoelectron spectroscopy (XPS)), Scanning Electron Microscopy (SEM) and EDS (energy-dispersive spectrometer) for the applications of chemical analysis, biochemistry, biophysics, and chemistry of surface material. |
| Prerequisites |
|
self-directed learning in the course |
Y |
| Relevance of Course Objectives and Core Learning Outcomes(%) |
Teaching and Assessment Methods for Course Objectives |
| Course Objectives |
Competency Indicators |
Ratio(%) |
Teaching Methods |
Assessment Methods |
The main objective of this course is how to apply the concepts of optical spectroscopy to investigate and characterize the molecules, cell biology, biophysics, and biochemistry.
|
| 1.Possess professional knowledge in biomedical engineering. |
| 2.Plan and implement research projects, and have the ability to solve problems independently. |
| 3.Ability to write scientific papers and communicate research results effectively. |
| 4.Integration in interdisciplinary research and innovative research skills. |
| 5.Possess insightful perspective on industry and globalization. |
|
|
| Lecturing |
| Discussion |
| Exercises |
| topic Discussion/Production |
| Networking/Distance Education |
|
| Quiz |
| Assignment |
| Oral Presentation |
| Attendance |
| Written Presentation |
|
| Course Content and Homework/Schedule/Tests Schedule |
| Week |
Course Content |
| Week 1 |
1. Introduction |
| Week 2 |
2. The Beer-Lambert Law
|
| Week 3 |
3. Absorption Spectra of Mixtures, Distortions of Absorption Spectra by Light Scattering or Nonuniform Distributions of the Absorbing Molecules |
| Week 4 |
4. UV/VIS spectra
|
| Week 5 |
5. Vibrational Adsorption
|
| Week 6 |
6. Absorption Spectra of Proteins and Nucleic Acids
|
| Week 7 |
7. IR spectra
|
| Week 8 |
8. Techniques for Measuring Absorbance
|
| Week 9 |
9. Introduction of the Photoelectric Effect
|
| Week 10 |
10.Lasers
|
| Week 11 |
11.Raman spectra, ESCA spectra, and AES spectra
|
| Week 12 |
12.Analysis of ESCA spectra by XPS peak software
|
| Week 13 |
13.Fluorescence – (Fluorescence Yields and Lifetimes, Photobleaching, Fluorescent Probes and Tags)
|
| Week 14 |
14.Single-Molecule, High-Resolution Microscopy, Scanning Electron Microscopy (SEM) and EDS (energy-dispersive spectrometer)
|
| Week 15 |
15.Final Exam
|
| Week 16 |
16.Project – Paper Reading and Discussion
|
| Week 17 |
17.Project – Paper Reading and Discussion
|
| Week 18 |
18.Final Report |
|
| Evaluation |
Attendance 5%, Homework 15%, Midterm 25%, Final Exam 25%, Oral presentation 15%,Final Report 15%
|
| Textbook & other References |
1. Modern Optical Spectroscopy: With Exercises and Examples from Biophysics and Biochemistry, Springer, by William W. Parson, 2nd printing edition, 2009.2.
2. Photoelectron Spectroscopy: Principles and Applications, Springer Berlin Heidelberg, by Stephan Hüfner, 2010.
|
| Teaching Aids & Teacher's Website |
| iLearning |
| Office Hours |
splin@dragon.nchu.edu.tw
Wednesday, 1:00 pm-3:00 pm
|
| Sustainable Development Goals, SDGs |
| 04.Quality Education | include experience courses：N |
|