| Relevance of Course Objectives and Core Learning Outcomes(%) |
Teaching and Assessment Methods for Course Objectives |
| Course Objectives |
Competency Indicators |
Ratio(%) |
Teaching Methods |
Assessment Methods |
| Research of public health and epidemiology requires a large number of laboratory techniques and diagnostic methods. Therefore, the purpose of this course is to equip students the principles and applications of common and novel laboratory diagnostic techniques. This lecture will help students to understand the diagnosis of infectious diseases and disease control. |
|
|
| Lecturing |
| Discussion |
| topic Discussion/Production |
| Exercises |
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| Attendance |
| Written Presentation |
| Assignment |
|
| Course Content and Homework/Schedule/Tests Schedule |
| Week |
Course Content |
| Week 1 |
Immunoassays (I): Immunoblot analysis and Immunofluorescence assay (IFA) |
| Week 2 |
Immunoassays (II): ELISA and hemagglutination (HA) |
| Week 3 |
Immunoassays (III): lateral flow device assay (LFA) |
| Week 4 |
Preparation of monoclonal antibody and therapeutic antibody |
| Week 5 |
Nucleotide amplification-based methods (I) |
| Week 6 |
Nucleotide amplification-based methods (II) |
| Week 7 |
Nucleotide amplification-based methods (III) |
| Week 8 |
Allele-Specific Mutation Detection by PCR-ARMS |
| Week 9 |
Mid-term Discussion (techniques selected for self-practice) |
| Week 10 |
Oligonucleotide Ligation Assays for the Diagnosis of Inherited Diseases |
| Week 11 |
isothermal amplification (I): NASBA |
| Week 12 |
isothermal amplification (II): Loop-mediated isothermal amplification (LAMP) |
| Week 13 |
isothermal amplification (III): rolling circle amplification (RCA) |
| Week 14 |
isothermal amplification (III): Recombinase polymerase amplification (RPA) |
| Week 15 |
isothermal amplification (IV): helicase-dependent amplification (HDA) |
| Week 16 |
Panel Discussion (techniques selected for self-practice)
Design and conduct one selected serological method. (2-page Report required)
Design and conduct one selected nucleic acid-based diagnostic method. (2-page Report required) |
self-directed
learning |
|
|
| Evaluation |
1. attendence
2. presentation and reports |
| Textbook & other References |
1. Molecular Diagnostics 3rd Edition, 2016
Editors: George Patrinos Wilhelm Ansorge Phillip B. Danielson
2.2. Diagnostic Microbiology. 1998. 10th edition. Edited by B.Forbes, D. Sahm, and A. Weissfeld.
3. Clinical Diagnosis and Management by Laboratory Methods. (1996).19th edition. Edited by J. B. Henry.
4. Analysis of Genes and Genomes. 2004. 1st edtion. Edited by R. J. Reece. Published by Wiley&Sons, Ltd. |
| Teaching Aids & Teacher's Website |
| i-learning |
| Office Hours |
| Friday afternoon |
| Sustainable Development Goals, SDGs(Link URL) |
| 03.Good Health and Well-Being | include experience courses：Y |
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