NCHU Course Outline
Course Name (中) 實驗診斷技術(6492)
(Eng.) Laboratory Diagnostic Technique
Offering Dept Graduate Institute of Microbiology and Public Health
Course Type Elective Credits 3 Teacher HSU,WEI-LI
Department Graduate Institute of Microbiology and Public Health/Graduate Language English Semester 2025-SPRING
Course Description The course introduces newly developed technologies including but not limited serological based methods and nucleic acid amplification methods. The principles, experimental design, and clinical applications will be introduced.
Prerequisites
self-directed learning in the course Y
Relevance of Course Objectives and Core Learning Outcomes(%) Teaching and Assessment Methods for Course Objectives
Course Objectives Competency Indicators Ratio(%) Teaching Methods Assessment Methods
Research of public health and epidemiology requires a large number of laboratory techniques and diagnostic methods. Therefore, the purpose of this course is to equip students the principles and applications of common and novel laboratory diagnostic techniques. This lecture will help students to understand the diagnosis of infectious diseases and disease control.
Lecturing
Discussion
topic Discussion/Production
Exercises
Attendance
Written Presentation
Assignment
Course Content and Homework/Schedule/Tests Schedule
Week Course Content
Week 1 Immunoassays (I): Immunoblot analysis and Immunofluorescence assay (IFA)
Week 2 Immunoassays (II): ELISA and hemagglutination (HA)
Week 3 Immunoassays (III): lateral flow device assay (LFA)
Week 4 Preparation of monoclonal antibody and therapeutic antibody
Week 5 Nucleotide amplification-based methods (I)
Week 6 Nucleotide amplification-based methods (II)
Week 7 Nucleotide amplification-based methods (III)
Week 8 Allele-Specific Mutation Detection by PCR-ARMS
Week 9 Mid-term Discussion (techniques selected for self-practice)
Week 10 Oligonucleotide Ligation Assays for the Diagnosis of Inherited Diseases
Week 11 isothermal amplification (I): NASBA
Week 12 isothermal amplification (II): Loop-mediated isothermal amplification (LAMP)
Week 13 isothermal amplification (III): rolling circle amplification (RCA)
Week 14 isothermal amplification (III): Recombinase polymerase amplification (RPA)
Week 15 isothermal amplification (IV): helicase-dependent amplification (HDA)
Week 16 Panel Discussion (techniques selected for self-practice)
Week 17 Design and conduct one selected serological method. (2-page Report required)
Week 18 Design and conduct one selected nucleic acid-based diagnostic method. (2-page Report required)
Evaluation
1. attendence
2. presentation and reports
Textbook & other References
1. Molecular Diagnostics 3rd Edition, 2016
Editors: George Patrinos Wilhelm Ansorge Phillip B. Danielson
2.2. Diagnostic Microbiology. 1998. 10th edition. Edited by B.Forbes, D. Sahm, and A. Weissfeld.
3. Clinical Diagnosis and Management by Laboratory Methods. (1996).19th edition. Edited by J. B. Henry.
4. Analysis of Genes and Genomes. 2004. 1st edtion. Edited by R. J. Reece. Published by Wiley&Sons, Ltd.
Teaching Aids & Teacher's Website
i-learning
Office Hours
Friday afternoon
Sustainable Development Goals, SDGs
03.Good Health and Well-Beinginclude experience courses：Y
Please respect the intellectual property rights and use the materials legally.Please repsect gender equality.
Update Date, year/month/day：None Printed Date, year/month/day：2025 / 1 / 22
The second-hand book website：http://www.myub.com.tw/