| Course Name |
(中) 醫用生物技術(一)(6701) |
| (Eng.) Medical Biotechnology(I) |
| Offering Dept |
Graduate Institute of Biomedical Sciences |
| Course Type |
Elective |
Credits |
2 |
Teacher |
CHUEH, PIN JU |
| Department |
Graduate Institute of Biomedical Sciences/Graduate |
Language |
English |
Semester |
2024-FALL |
| Course Description |
It is important to understand the basis for common biotechnology in order to perform the techniques correctly. Therefore, in this course, we introduce several techniques that are routinely used in biomedical-related research, including cell culture, cytotoxicity assays, applications of flow cytometry, transfection, transformation, Western blot analysis, DNA/RNA analysis. |
| Prerequisites |
|
self-directed learning in the course |
N |
| Relevance of Course Objectives and Core Learning Outcomes(%) |
Teaching and Assessment Methods for Course Objectives |
| Course Objectives |
Competency Indicators |
Ratio(%) |
Teaching Methods |
Assessment Methods |
| We anticipate that students who take this course have the ability to conduct those experiments correctly and safely. |
|
|
| Lecturing |
| Practicum |
| Discussion |
| topic Discussion/Production |
|
| Internship |
| Oral Presentation |
| Attendance |
|
| Course Content and Homework/Schedule/Tests Schedule |
| Week |
Course Content |
| Week 1 |
Introduction |
| Week 2 |
Cell culture |
| Week 3 |
Cytotoxicity |
| Week 4 |
Flow Cytometry and Apoptosis |
| Week 5 |
ROS determination |
| Week 6 |
Cell Cycle Determination |
| Week 7 |
Apoptosis Determination |
| Week 8 |
Transformation |
| Week 9 |
Transfection |
| Week 10 |
Plasmid Purification |
| Week 11 |
Protein sample preparation |
| Week 12 |
SDS-PAGE/Coomassie blue staining |
| Week 13 |
SDS-PAGE/Gel transfer/Western Blot Analysis |
| Week 14 |
Film exposure for Western Blot Analysis |
| Week 15 |
RNA purification |
| Week 16 |
RT-PCR |
| Week 17 |
DNA gel electrophoresis |
| Week 18 |
Final Report |
|
| Evaluation |
Attendance 30%
Performance in class 60%
Oral final report 10% |
| Textbook & other References |
Lecture notes on iLearning system
|
| Teaching Aids & Teacher's Website |
iLearning system
|
| Office Hours |
| Monday afternoons |
| Sustainable Development Goals, SDGs |
| | include experience courses：N |
|