Course Name |
(中) 醫用生物技術(一)(6701) |
(Eng.) Medical Biotechnology(I) |
Offering Dept |
Graduate Institute of Biomedical Sciences |
Course Type |
Elective |
Credits |
2 |
Teacher |
CHUEH, PIN JU |
Department |
Graduate Institute of Biomedical Sciences/Graduate |
Language |
English |
Semester |
2024-FALL |
Course Description |
It is important to understand the basis for common biotechnology in order to perform the techniques correctly. Therefore, in this course, we introduce several techniques that are routinely used in biomedical-related research, including cell culture, cytotoxicity assays, applications of flow cytometry, transfection, transformation, Western blot analysis, DNA/RNA analysis. |
Prerequisites |
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self-directed learning in the course |
N |
Relevance of Course Objectives and Core Learning Outcomes(%) |
Teaching and Assessment Methods for Course Objectives |
Course Objectives |
Competency Indicators |
Ratio(%) |
Teaching Methods |
Assessment Methods |
We anticipate that students who take this course have the ability to conduct those experiments correctly and safely. |
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|
Lecturing |
Practicum |
Discussion |
topic Discussion/Production |
|
Internship |
Oral Presentation |
Attendance |
|
Course Content and Homework/Schedule/Tests Schedule |
Week |
Course Content |
Week 1 |
Introduction |
Week 2 |
Cell culture |
Week 3 |
Cytotoxicity |
Week 4 |
Flow Cytometry and Apoptosis |
Week 5 |
ROS determination |
Week 6 |
Cell Cycle Determination |
Week 7 |
Apoptosis Determination |
Week 8 |
Transformation |
Week 9 |
Transfection |
Week 10 |
Plasmid Purification |
Week 11 |
Protein sample preparation |
Week 12 |
SDS-PAGE/Coomassie blue staining |
Week 13 |
SDS-PAGE/Gel transfer/Western Blot Analysis |
Week 14 |
Film exposure for Western Blot Analysis |
Week 15 |
RNA purification |
Week 16 |
RT-PCR |
Week 17 |
DNA gel electrophoresis |
Week 18 |
Final Report |
|
Evaluation |
Attendance 30%
Performance in class 60%
Oral final report 10% |
Textbook & other References |
Lecture notes on iLearning system
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Teaching Aids & Teacher's Website |
iLearning system
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Office Hours |
Monday afternoons |
Sustainable Development Goals, SDGs |
  | include experience courses:N |
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