NCHU Course Outline
Course Name (中) 農桿菌之分子致病機制導論(6576)
(Eng.) Introductions of Molecular Mechanisms of Agrobacterium-mediated Plant
Offering Dept Department of Life Sciences
Course Type Elective Credits 2 Teacher HWANG, HAU-HSUAN
Department Department of Life Sciences/Graduate Language 中/英文 Semester 2025-SPRING
Course Description Topics include: 1. The initial steps in Agrobacterium tumefaciens pathogenesis; 2. Agrobacterium-host attachment; 3. Production of a mobile T-DNA by Agrobacterium tumefaciens; 4. Translocation of oncogenic T-DNA and effector proteins to plant cells; 5. Intracellular transport of Agrobacterium T-DNA; 6. Function of host proteins in the Agrobacterium-mediated plant transformation process; 7. Agrobacterium-mediated transformation of non-plant organisms
Prerequisites
self-directed learning in the course N
Relevance of Course Objectives and Core Learning Outcomes(%) Teaching and Assessment Methods for Course Objectives
Course Objectives Competency Indicators Ratio(%) Teaching Methods Assessment Methods
5.(乙組)瞭解生物生理機能
6.(乙組)瞭解生物發生的現象及調控機制

5.Realizing the physiological mechanisms of the organisms
6.Realizing the development and regulatory mechanisms of the organisms
50
50
Lecturing
Discussion
Quiz
Oral Presentation
Course Content and Homework/Schedule/Tests Schedule
Week Course Content
Week 1 1. Introduction of class

Week 2 2. The initial steps in Agrobacterium tumefaciens pathogenesis Agrobacterium: From Biology to Biotechnology (Chap 6)
Week 3 3. The initial steps in Agrobacterium tumefaciens pathogenesis Selected Papers
Week 4 4. Agrobacterium-host attachment Agrobacterium: From Biology to Biotechnology (Chap 7)
Week 5 5. Agrobacterium-host attachment Selected Papers
Week 6 6. Production of a mobile T-DNA by Agrobacterium tumefaciens Agrobacterium: From Biology to Biotechnology (Chap 8)

Week 7 7. Production of a mobile T-DNA by Agrobacterium tumefaciens Selected Papers
Week 8 8. Translocation of oncogenic T-DNA and effector proteins to plant cells Agrobacterium: From Biology to Biotechnology (Chap 9)
Week 9 9. Translocation of oncogenic T-DNA and effector proteins to plant cells Selected Papers
Week 10 10. Translocation of oncogenic T-DNA and effector proteins to plant cells Selected Papers

Week 11 11. Intracellular transport of Agrobacterium T-DNA Agrobacterium: From Biology to Biotechnology (Chap 10)
Week 12 12. Intracellular transport of Agrobacterium T-DNA Selected Papers
Week 13 13. Intracellular transport of Agrobacterium T-DNA Selected Papers

Week 14 14. Function of host proteins in the Agrobacterium-mediated plant transformation process Agrobacterium: From Biology to Biotechnology (Chap 13)
Week 15 15. Function of host proteins in the Agrobacterium-mediated plant transformation process Selected Papers
Week 16 16. Function of host proteins in the Agrobacterium-mediated plant transformation process Selected Papers
Week 17 17. Agrobacterium-mediated transformation of non-plant organisms Agrobacterium: From Biology to Biotechnology (Chap 18)

Week 18 18. Final Exam (30% of total grade)
Evaluation
Paper discussion and presentation 80%, Exam 20%
Textbook & other References
1. Tzfira, T., and Citovsky, V. (Eds.). 2008. Agrobacterium: From Biology to Biotechnology. Springer Science and Business Media, New York, NY, USA.
2. Chrispeels, M.J, and Sadava, D.E. 2003. Plants, Genes, and Crop Biotechnology. Jones and Bartlett Publishers, Sudbury, MA, USA.
3. Wang, K. (Eds). 2006. Agrobacterium Protocols. Humana Press, Totowa, NJ, USA.
4. Selected papers.
Teaching Aids & Teacher's Website
iLearning
Office Hours
另行公布
Sustainable Development Goals, SDGs
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Update Date, year/month/day:None Printed Date, year/month/day:2025 / 1 / 22
The second-hand book website:http://www.myub.com.tw/