NCHU Course Outline

Course Name	(中) 作物育種學特論(8064)
	(Eng.) Special Topics in Crop Breeding
Offering Dept	Department of Agronomy
Course Type	Elective	Credits	3	Teacher	KU,HSIN-MEI
Department	Department of AgronomyPh.D	Language	English	Semester	2024-FALL
Course Description	建議修過高等遺傳學,植物生物技術及分子生物及植物病毒學相關課程再選修本課程; 本課程介紹VIGE研究之理論及實例;This course introduces students to be familiar with the theory and methods of Special Topics in Crop Breeding using studies of VIGE as case studies. Students could also learn about the logical thinking of science underlying individual experiments with the in-class teacher-student interaction-based method.
Prerequisites				self-directed learning in the course	Y

Relevance of Course Objectives and Core Learning Outcomes(%)			Teaching and Assessment Methods for Course Objectives
Course Objectives	Competency Indicators	Ratio(%)	Teaching Methods	Assessment Methods
具備分子遺傳育種學及分子技術應用於作物改良之知識。	1. 2. 3. 4. 5. 6.	20 40 10 10 10 10	Discussion Lecturing	Written Presentation Assignment
Course Content and Homework/Schedule/Tests Schedule
Week Course Content Week 1 Mahmood 2023 Plant Virus-Derived Vectors for Plant Genome Engineering. Viruses 15:531; Cody, W. B. & Scholthof, H. B. 2019 Plant virus vectors 3.0: transitioning into synthetic genomics. Annu. Rev. Phytopathol. 57, 211–230 (2019). Week 2 Liu et al 2023 Engineered biocontainable RNA virus vectors for nontransgenic genome editing across crop species and genotypes. Mol Plant 16, 616–631. Week 3 Chao 2024 Efficient and transformation‐free genome editing in pepper enabled by RNA virus‐mediated delivery of CRISPR/Cas9. J integrative Plant Biology DOI:10.1111/jipb.13741 Week 4 Lee et al 2024 Development of virus-induced genome editing methods in Solanaceous crops. Horticulture Research 11: uhad233 Week 5 Ma et al 2020 Highly efficient DNA-free plant genome editing using virally delivered CRISPR-Cas9. Nature plants 6:773-779 Week 6 Tang 2024 Genome editing in rice and tomato with a small Un1Cas12f1 nuclease. Plant Genome. 2024;17:e20465. Week 7 Gong et al 2024 Geminiviral-induced genome editing using miniature CRISPR/Cas12j (CasΦ) and Cas12f variants in plants. Plant Cell Reports 43:71 Week 8 自主學習: students would review courses taught and discuss with classmates and the teacher. Week 9 midterm exame Week 10 wang 2024 Efficient tobacco rattle virus-induced gene editing in tomato mediated by the CRISPR/Cas9 system Biotechnology J. 19: e2400204. doi: 10.1002/biot.202400204. Week 11 Chen et al 2022 Development and optimization of a Barley stripe mosaic virus-mediated gene editing system to improve Fusarium head blight resistance in wheat. Plant Biotechnol. J. 20:1018–1020. Week 12 Beernink BM, Lappe RR, Bredow M and Whitham SA (2022) Impacts of RNA Mobility Signals on Virus Induced Somatic and Germline Gene Editing. Front. Genome Ed. 4:925088. Week 13 Chiong et al 2021 RNA silencing suppressor infuenced performance of a virus vector delivering both guide RNA and Cas9 for CRISPR gene editing. Scientific Reports 11: 6769 Week 14 Uranga et al 2024 RNA virus-mediated gene editing for tomato trait breeding. Horticulture Research 11:uhad279 Week 15 Zhang 2023 A combinatorial TRM-OFP module bilaterally fine-tunes tomato fruit shape New Phytologist (2023) 238: 2393–2409 doi: 10.1111/nph.18855 Week 16 Naeem et al 2023 Beyond green and red: unlocking the genetic orchestration of tomato fruit color and pigmentation. Funct Inter Genet 23:243 https://link.springer.com/article/10.1007/s10142-023-01162-5 Week 17 自主學習: students would review courses taught and discuss with classmates and the teacher. Week 18 final exame
Evaluation
discussion 40% reports 60%
Textbook & other References
1. references as the listed 2. Weaver 2008 Molecular Biology 4ed The McGraw Hill companies ISBN 9780071102167 3.Klug 2010 Concepts of Genetics (9ed) Pearson/Benjamin Cummings, New York , USA, ISBN 0321540980歐亞書局 Eurasia Book Co.
Teaching Aids & Teacher's Website
Office Hours
Thursday 4-5 pm
Sustainable Development Goals, SDGs
include experience courses：N

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Update Date, year/month/day：2024/09/12 14:48:25	Printed Date, year/month/day：2024 / 11 / 21
The second-hand book website：http://www.myub.com.tw/