| Relevance of Course Objectives and Core Learning Outcomes(%) |
Teaching and Assessment Methods for Course Objectives |
| Course Objectives |
Competency Indicators |
Ratio(%) |
Teaching Methods |
Assessment Methods |
學生們將於此實習課程中，學習與操作到分生相關實驗技術，也將會於課堂中討論各種新的分生技術與其在植物病理鑑定上的應用。
Students will study molecular techniques for plant disease diagnosis. We will discuss the new techniques in the class. |
| 2.To develop the abilities of plant clinical diagnosis and prescribing pesticides |
| 3.Techniques and knowledge of plant quarantine and disease management |
| 5.Development of the ability to conduct scientific research, to work in a team and to present and interpret scientific results |
|
|
| Lecturing |
| Practicum |
| topic Discussion/Production |
|
| Internship |
| Oral Presentation |
|
| Course Content and Homework/Schedule/Tests Schedule |
| Week |
Course Content |
| Week 1 |
Introduction |
| Week 2 |
Collect disease sample/Isolate pathogen |
| Week 3 |
DNA extraction |
| Week 4 |
DNA extraction and ITS PCR |
| Week 5 |
Analyze ITS sequence and molecular cloning_Ligation |
| Week 6 |
Molecular cloning_E.coli Transformation |
| Week 7 |
Molecular cloning_Plasmid extraction |
| Week 8 |
RNA extraction |
| Week 9 |
RT-PCR |
| Week 10 |
design qPCR primers and microbial genome search |
| Week 11 |
qPCR /Submit lab notebook and the title of your report. |
| Week 12 |
LAMP PCR |
| Week 13 |
Microscope |
| Week 14 |
Protein extraction and SDS PAGE |
| Week 15 |
ELISA |
| Week 16 |
Immunostrip |
| Week 17 |
Group presentation |
| Week 18 |
Submit lab notebook |
|
| Evaluation |
出席狀況(30%)、口頭報告(30%)、實習參與(40%)
Attendance (30%), Oral presentation (30%) and experiment (40%) |
| Textbook & other References |
|
| Teaching Aids & Teacher's Website |
| Power point presentation |
| Office Hours |
| Please directly contact the instructor. |
| Sustainable Development Goals, SDGs |
| 02.Zero Hunger 03.Good Health and Well-Being | include experience courses：N |
|