NCHU Course Outline
Course Name (中) 分子植物科學技術實作(6935)
(Eng.) Current Molecular Plant Science Technology in Practices
Offering Dept Master Program in Plant Health Care
Course Type Elective Credits 2 Teacher Tao-Ho Chang
Department Master Program in Plant Health Care/Graduate Language English Semester 2025-SPRING
Course Description The emerging area of molecular plant technology determines the shape of modern agriculture. This course aims to introduce current plant science technology. The course will include the practices on the model plant Arabidopsis. Additionally, this course will lead students to discover the novel technology happening in the current world.
Prerequisites
self-directed learning in the course N
Relevance of Course Objectives and Core Learning Outcomes(%) Teaching and Assessment Methods for Course Objectives
Course Objectives Competency Indicators Ratio(%) Teaching Methods Assessment Methods
1.Introduction of the current plant science
2.Understanding the concept of these novel technology
3.Practices on model plants
1.
2.
3.
50
25
25
topic Discussion/Production
Exercises
Discussion
Lecturing
Attendance
Oral Presentation
Study Outcome
Course Content and Homework/Schedule/Tests Schedule
Week Course Content
Week 1 Arabidopsis cultivation: grow your own Arabidopsis plants
Week 2 Bioinformatic: target sequence selection and primers design
Week 3 Arabidopsis DNA extraction
Week 4 Arabidopsis RNA extraction
Week 5 Polymerase chain reaction and qRT-PCR
Week 6 Arabidopsis cultivation: collect your own Arabidopsis seeds stock
Week 7 DNA and RNA electrophoresis
Week 8 Arabidopsis Protein extraction
Week 9 Mid-term
Week 10 Arabidopsis-microbe interaction analysis1: Arabidopsis plating analysis
Week 11 Arabidopsis-microbe interaction analysis2: Arabidopsis root system architecture
Week 12 Arabidopsis-microbe interaction analysis3: Redox enzymes activity
Week 13 Arabidopsis-microbe interaction analysis4: Arabidopsis defence response
Week 14 Arabidopsis-microbe interaction analysis5: Bacteria pathogen infiltration
Week 15 Arabidopsis-microbe interaction analysis6: Redox image analysis
Week 16 Generating Arabidopsis mutant: pollinated mutant lines
Week 17 Generating Arabidopsis mutant: flower dipping
Week 18 Final remarks and oral reports
Evaluation
Group discussion participation (30%)
Presentation (20%)
Practices output (50%)
Textbook & other References
Weigel, D., and Glazebrook, J. (2002). Arabidopsis: A Laboratory Manual. CSHL Press.
The Arabidopsis Information Resources. https://www.arabidopsis.org/
Teaching Aids & Teacher's Website

Office Hours
Make reservation through email
Sustainable Development Goals, SDGs
 include experience courses：N
Please respect the intellectual property rights and use the materials legally.Please repsect gender equality.
Update Date, year/month/day：None Printed Date, year/month/day：2025 / 1 / 22
The second-hand book website：http://www.myub.com.tw/