| 週次 |
授課內容 |
| 第1週 |
1.Ratcliff et al 2001 Tobacco rattle virus as a vector for analysis of gene function by silencing. The Plant Journal 25:237-245; Rossner C, Lotz D, and Becker A 2022 VIGS goes viral: How VIGS transforms our understanding of plant science. Ann Rev Plant Biol 73: (pp703-716) |
| 第2週 |
2.Senthil-Kumar et al 2007 A systematic study to determine the extent of gene silencing in Nicotiana benthamiana and other Solanaceae species when heterologous gene sequences are used for virus-induced gene silencing. New Phytologist 176: 782–791 |
| 第3週 |
3.Shi et al 2021 A Methodological Advance of Tobacco Rattle Virus-Induced Gene Silencing for Functional Genomics in Plants. Front. Plant Sci. 12:671091. doi: 10.3389/fpls.2021.671091; |
| 第4週 |
4.Zhang 2020 Genome editing with the CRISPR-Cas system: an art ethics and global regulatory perspective. Plant biotechnol J 18: 1651-1669; Awan 2023 Viral vectors as carriers of genome-editing reagents. Trends in Plant Science 28: 981-983; Ali 2015 Efficient Virus-Mediated Genome Editing in Plants Using the CRISPR/Cas9 System. Molecular Plant 8, 1288–1291 (ref Zhang 2022 Virus-Induced Gene Editing and Its Applications in Plants. Int. J. Mol. Sci. 23, 10202. https://doi.org/10.3390/ ijms231810202) |
| 第5週 |
5.Tran, M. T., Doan, D. T. H., Kim, J., Song, Y. J., Sung, Y. W., Das, S., et al. (2021). CRISPR/Cas9-based precise excision of SlHyPRP1 domain(s) to obtain salt stress-tolerant tomato. Plant Cell Rep. 40, 999–1011. doi: 10.1007/s00299-020-02622-z |
| 第6週 |
6.Liu, L., Zhang, J., Xu, J., Li, Y., Guo, L., Wang, Z., et al. (2020). CRISPR/Cas9 targeted mutagenesis of SlLBD40, a lateral organ boundaries domain transcription factor, enhances drought tolerance in tomato. Plant Sci. 301, 110683. doi: 10.1016/ j.plantsci.2020.110683; |
| 第7週 |
7.自主學習: 應與老師或同學進行討論並應研讀VIGE之理論及方法並進行整理。 |
| 第8週 |
8.期中考週 |
| 第9週 |
9.Wang, P., Luo, Q., Yang, W., Ahammed, G. J., Ding, S., Chen, X., et al. (2021). A novel role of pipecolic acid biosynthetic pathway in drought tolerance through the antioxidant system in tomato. Antioxidants (Basel) 10 (12), 1923. doi: 10.3390/antiox10121923 |
| 第10週 |
10.Zhang 2018 Suppressing type 2C protein phosphatases alters fruit ripening and the stress response in tomato. Plant Cell Physiol. 2018, 59, 142–154.drought |
| 第11週 |
11.Sun, 2017 Suppressing ABA uridine diphosphate glucosyltransferase (Sl UGT 75C1) alters fruit ripening and the stress response in tomato. Plant J. 91, 574–589 drought |
| 第12週 |
12.Yin, W.; Hu, Z.; Cui, B.; Guo, X.; Hu, J.; Zhu, Z.; Chen, G. 2017 Suppression of the MADS-box gene SlMBP8 accelerates fruit ripening of tomato (Solanum lycopersicum). Plant Physiol. Biochem. 118, 235–244 Drought and salt |
| 第13週 |
13.Campos, J.F.; Cara, B.; Pérez-Martín, F.; Pineda, B.; Egea, I.; Flores, F.B.; Fernandez-Garcia, N.; Capel, J.; Moreno, V.; Angosto, T. 2016 The tomato mutant ars1 (altered response to salt stress 1) identifies an R1-type MYB transcription factor involved in stomatal closure under salt acclimation. Plant Biotechnol. J. 14, 1345–1356. |
| 第14週 |
14.Cai, X.; Zhang, C.; Shu, W.; Ye, Z.; Li, H.; Zhang, Y. 2016 The transcription factor SlDof22 involved in ascorbate accumulation and salinity stress in tomato. Biochem. Biophys. Res. Commun. 2016, 474, 736–741 |
| 第15週 |
15.Yang, Y.; Tang, N.; Xian, Z.; Li, Z. 2015 Two SnRK2 protein kinases genes play a negative regulatory role in the osmotic stress response in tomato. Plant Cell Tissue Organ Cult. PCTOC 122, 421–43 |
| 第16週 |
16.Xu, X.-X.; Hu, Q.; Yang, W.-N.; Jin, Y. 2017 The roles of cell wall invertase inhibitor in regulating chilling tolerance in tomato. BMC Plant Biol. 17, 195. |
| 第17週 |
17.自主學習: 應與老師或同學進行討論並應研讀耐逆境之理論及思考如何以VIGE策略設計試驗，進行思考及整理。 |
| 第18週 |
期末考週 |
|