NCHU Course Outline
Course Name (中) 植物基因編輯技術:原理與應用(6341)
(Eng.) Advanced Plant Gene Editing: Principles and Applications
Offering Dept Graduate Institute of Biotechnology
Course Type Elective Credits 2 Teacher HUANG YING-WEN
Department Graduate Institute of Biotechnology / Graduate Language Chinese Semester 2025-FALL
Course Description 本課程探討基因編輯技術及其在植物科學中的應用。學生將探索主要基因編輯的運作機制、實驗設計、基因導入方法以及在農業和生物技術中的實際應用。課程強調實作學習及對該領域最新進展的了解與分析。
This course provides an understanding of gene editing technologies and their applications in plant science. Students will explore the mechanisms of major gene-editing tools, experimental design, delivery methods, and real-world applications in agriculture and biotechnology. The course emphasizes hands-on learning and critical analysis of recent advancements in the field
Prerequisites
Relevance of Course Objectives and Core Learning Outcomes(%) Teaching and Assessment Methods for Course Objectives
Course Objectives Competency Indicators Ratio(%) Teaching Methods Assessment Methods
本課程目的在培養學生對植物基因編輯技術的基礎原理與應用能力,涵蓋CRISPR/Cas9等工具的操作原理、實驗設計與實作應用,並強調在作物改良與永續農業中的實際應用潛力。
Lecturing
topic Discussion / Production
Exercises
Discussion
Practicum
Written Presentation
Attendance
Oral Presentation
Assignment
Quiz
Internship
Course Content and Homework/Schedule/Tests Schedule
Week Course Content
Week 1 課程介紹與植物基因編輯概述 (Introduction to Plant Gene Editing)
Week 2 植物基因體學基礎知識與主要基因編輯技術總覽 (Genome Organization of Plants and Overview of Major Gene Editing Tools)
Week 3 CRISPR/Cas9 技術原理與應用 (CRISPR/Cas9: Mechanisms and Applications)
Week 4 其他先進基因編輯系統 (Other Gene Editing Tools)
Week 5 基因編輯實驗設計與目標選擇 (Designing Gene Editing Experiments)
Week 6 植物基因導入方法與轉殖技術 (Gene Delivery Methods in Plants)
Week 7 基因編輯結果的檢測與分析技術 (Experimental Controls and Data Analysis Methods)
Week 8 期中考
Week 9 植物基因編輯在作物改良中的應用 I (Applications in Crop Improvement I)
Week 10 植物基因編輯在作物改良中的應用 II (Applications in Crop Improvement II)
Week 11 多倍體植物的基因編輯挑戰與策略 (Gene Editing Challenges and Strategies in Polyploid Plants)
Week 12 基因功能剖析:基因敲除與替換技術 (Gene Function Analysis: Knockout and Replacement Techniques)
Week 13 基因編輯結果的評估與未來展望 (Evaluation of Gene Editing Results and Future Perspectives)
Week 14 實作專題設計 I — 小組專題指導 (Hands-on Project Design I)
Week 15 實作專題設計 II — 學生專題成果發表 (Hands-on Project Design II)
Week 16 期末考
self-directed
learning
   01.Participation in professional forums, lectures, and corporate sharing sessions related to industry-government-academia-research exchange activities.
   02.Viewing multimedia materials related to industry and academia.
   03.Preparing presentations or reports related to industry and academia.
   05.Participation in various workshops organized by different departments of NCHU.

Evaluation
平時作業與文獻探討(20%); 實驗報告與實作成果(30%); 期中考試(20%); 期末專題報告與期末考(30%)
Textbook & other References
Genome Editing in Plants (Editors: Om Prakash Gupta, Suhas Gorakh Karkute; Publisher: Taylor & Francis; ISBN 9780367815370)
Plant Genome Editing Technologies (Editors: Jen-Tsung Chen, Sunny Ahmar; Publisher: Springer Singapore; DOI https://doi.org/10.1007/978-981-99-9338-3)
Plant Genome Engineering (Editors: Bing Yang, Wendy Harwood, Qiudeng Que; Publisher: Humana New York, NY; DOI https://doi.org/10.1007/978-1-0716-3131-7)
Teaching Aids & Teacher's Website
教師講義
Office Hours
每週二下午2:00至4:00(或依實際課程進度調整)
Sustainable Development Goals, SDGs(Link URL)
02.Zero Hunger   03.Good Health and Well-Being   04.Quality Education   07.Affordable and Clean Energy   13.Climate Action   15.Life On Landinclude experience courses:Y
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Update Date, year/month/day:2025/08/06 11:42:55 Printed Date, year/month/day:2026 / 10 / 06
The second-hand book website:http://www.myub.com.tw/