NCHU Course Outline
Course Name (中) 生物化學實習(3253)
(Eng.) Biochemistry Laboratory
Offering Dept Department of Food Science and Biotechnology
Course Type Required Credits 2 Teacher TSAI,SHUO-WEN et al.
Department Department of Food Science and Biotechnology / Undergraduate Language Chinese Semester 2025-FALL
Course Description 本課程兼顧生物化學領域最重要的四個部份:共同基礎實驗、酵素動力學、核酸操作、蛋白質純化,分別由專精老師授課,由實驗中印證課本所學。,使學生了解生物化學相關實驗之原理並進行實作,訓練學生未來實際著手進行研究之能力。(強烈建議至少修過一個學期生物化學課程再來選修,較有收獲)
Prerequisites 生物化學
Relevance of Course Objectives and Core Learning Outcomes(%) Teaching and Assessment Methods for Course Objectives
Course Objectives Competency Indicators Ratio(%) Teaching Methods Assessment Methods
使學生了解生物化學相關實驗之原理並進行實作,訓練學生未來實際著手進行研究之能力。
1.Have knowledge and skill in food science and biotechnology
2.Understand the practice in food science and biotechnology
4.Train the ability of logic thinking, communication, and cooperation
60
20
20
Lecturing
Other
Practicum
Discussion
Other
Internship
Quiz
Assignment
Attendance
Written Presentation
Course Content and Homework/Schedule/Tests Schedule
Week Course Content
Week 1 分組、清點、基本技術
Week 2 胺基酸滴定曲線:緩衝現象及蛋白質等店點
Week 3 蛋白質濃度定量:染劑結合法
Week 4 旋光性質:光學活性
Week 5 進度調整
Week 6 還原糖定量:DNS法
Week 7 酵素反應趨勢
Week 8 酵素特性研究:最適溫度、最適pH
Week 9 微生物質體純化及DNA電泳
Week 10 質體的限制酶圖譜
Week 11 DNA片段放大技術:PCR
Week 12 山羊免疫球蛋白IgG之純化:色層分析1
Week 13 山羊免疫球蛋白IgG之純化:色層分析2
Week 14 蛋白質電泳
Week 15 操作考及期末筆試
Week 16 進度調整
self-directed
learning
本課程為二學分課程,學生應自主學習四小時,學習內容為教師指定自修章節,學習完成應於 iLearning 系統依要求繳交報告,才算完成自主學習。
Evaluation
書面實驗報告與作業,課堂表現與出席狀況,測驗等
Textbook & other References
自編教材、講義、網上教材等
Teaching Aids & Teacher's Website
iLearning 3.0
Office Hours
每學期第一次上課時與選修學生商討後訂定
Sustainable Development Goals, SDGs(Link URL)
04.Quality Educationinclude experience courses:N
Please respect the intellectual property rights and use the materials legally.Please respect gender equality.
Update Date, year/month/day:2025/09/29 14:59:17 Printed Date, year/month/day:2026 / 4 / 16
The second-hand book website:http://www.myub.com.tw/