NCHU Course Outline

Course Name	(中) 蛋白質純化和X光晶體繞射學(6416)
	(Eng.) Protein Purification and X-Ray Crystallography
Offering Dept	Department of Food Science and Biotechnology
Course Type	Elective			Credits	2	Teacher	Yeh Chen
Department	Department of Food Science and Biotechnology / Graduate			Language	Chinese	Semester	2025-FALL
Course Description	本課程旨在教導學生學習並掌握如何正確地表達及純化重組蛋白、如何培養蛋白質晶體以及如何收集X光繞射數據並解析其結構，使學生能夠掌握此研究生命科學不可或缺的一大利器
Prerequisites

Relevance of Course Objectives and Core Learning Outcomes(%)			Teaching and Assessment Methods for Course Objectives
Course Objectives	Competency Indicators	Ratio(%)	Teaching Methods	Assessment Methods
1.瞭解蛋白質結晶的基本原理To understand the basic principles of protein crystallography 2.瞭解如何收集並處理X光繞射數據To understand how to collect and process X-ray diffraction data 3.瞭解如何建立蛋白質的3D立體結構To understand how to build three-dimensional structure of protein			topic Discussion / Production Discussion	Oral Presentation Quiz
Course Content and Homework/Schedule/Tests Schedule
Week Course Content Week 1 介紹重組蛋白質技術 Introduction of Recombinant Protein Production Week 2 介紹不同的蛋白質表達系統_大腸桿菌及酵母菌系統 Introduction of E. coli and Yeast Expression Systems Week 3 介紹不同的蛋白質表達系統_昆蟲細胞及哺乳細胞 Introduction of Insect Cell and Mammalian Cell Expression Systems Week 4 融合蛋白技術 Fusion Protein Techniques Week 5 蛋白純化原理介紹 Basic Principles of Protein Purification Week 6 蛋白質純化技術與策略(一) Techniques and Strategies of Protein Purification Week 7 蛋白質純化技術與策略(二) Techniques and Strategies of Protein Purification Week 8 蛋白質純化技術與策略(三) Techniques and Strategies of Protein Purification Week 9 期中測驗 Mid-Term Exam Week 10 蛋白質晶體學概論 Introduction of Protein Crystallography Week 11 晶體對稱性 Crystal Symmetry Week 12 蛋白結晶原理 Protein Crystallization Experiments Week 13 蛋白結晶實驗 Protein Crystallization Experiments Week 14 X光繞射學 X-Ray Diffraction Week 15 相位角解析技術_分子取代法 Phasing Methods_Molecular Replacement Week 16 自主練習 autonomic learning 自主練習 autonomic learning 期末測驗 self-directed learning
Evaluation
期中、期末筆試，出席狀況，課堂參與表現
Textbook & other References
Teaching Aids & Teacher's Website
Office Hours
Sustainable Development Goals, SDGs(Link URL)
07.Affordable and Clean Energy   14.Life Below Water   15.Life On Land   17.Partnerships for the Goals include experience courses：N

Please respect the intellectual property rights and use the materials and protected electronic files legally. Please respect gender equality.
Update Date, year/month/day：None	Printed Date, year/month/day：2026 / 9 / 13
The second-hand book website：http://www.myub.com.tw/