國立中興大學教學大綱

課程名稱	(中) 農用分子生物技術與重組桿狀病毒表現系統(6937)
	(Eng.) Technology of Agricultural Molecular Biology and Recombinant Baculovirus System
開課單位	植保碩士學程
課程類別	選修	學分	3	授課教師	唐政綱
選課單位	植保博士學程 / 博士班	授課使用語言	中文	英文/EMI		開課學期	1132
課程簡述	Baculovirus expression system is one of the most important technics that developed from insect science. It has not only been loaded in textbook, but also become a useful tool in biotechnological industry. For example, in pharmaceutical industry, there are some famous products produced by this technic, such as insulin, cytokines, growth factors, etc. Recombinant protein expression is the necessary technology behind this growing industry. This course will introduce the different expression systems used for recombinant protein production, the latest technologies of protein display and protein purification, and the fundamentals of fermentation. The Laboratory section will include the cloning of target genes into the expression vectors, protein purification, characterizations of the expressive products, and developments of their applications.
先修課程名稱						課程含自主學習	N

課程與核心能力關聯配比(%)

課程目標之教學方法與評量方法

課程目標

核心能力

配比(%)

教學方法

評量方法

Students are expected to learn the theory, practice, and the technology of agricultural molecular biology and recombinant baculovirus system, which would assist them to join the field of biopharmaceuticals in the future.

1.具備農作物整合性健康管理高階專業知能

2.具備循環經濟與永續管理高階專業知能

3.具備科學研究、團隊合作與資訊推廣能力

4.具備友善環境及安全農業高階專業知能

專題探討/製作

習作

討論

實習

講授

書面報告

出席狀況

實作

其他

授課內容(單元名稱與內容、習作/每週授課、考試進度-共18週)

週次	授課內容
第1週	The course introduction and viewing the syllabus
第2週	History of recombinant protein technologies
第3週	Clinical applications: biopharmaceuticals and protein drugs
第4週	Protein expression systems: Prokaryotic
第5週	Protein expression systems: Eukaryotic (insect cells and mammalian cells)
第6週	Genetic engineering of each expression system
第7週	Protein surface display techniques
第8週	Protein purification: Separation, precipitation, centrifugation, and filtration of cells and proteins
第9週	Midterm report (期中考週)
第10週	Chromatographic techniques for protein purification
第11週	Design of a fermenter
第12週	Biosensors and process control
第13週	Cell line rearing: Sf9 and Sf21
第14週	Experiments: Cloning of the expression vectors for enzyme – 1
第15週	Experiments: Cloning of the expression vectors for enzyme - 2
第16週	Experiments: Recombinant protein purification
第17週	Experiments: Enzymatic assay
第18週	Final report (期末考週)

學習評量方式

課程分數與成績評量:
平時表現與出席: 40%
書面報告: 40%
實驗室實作: 20%

教科書＆參考書目(書名、作者、書局、代理商、說明)

教科書:
1. Production of Recombinant Proteins: Novel Microbial and Eukaryotic Expression Systems (Editor: Gerd Gellissen) (ISBN: 978-3-527-31036-4, January 2005, Wiley-Blackwell, 429 Pages)
參考書目:
1. Cell Culture Engineering: Recombinant Protein Production (Advanced Biotechnology) (Editor: Gyun-Min Lee et al.) (ISBN-13: 978-3527343348, January 2020, Wiley-VCH, 440 Pages)

課程教材（教師個人網址請列在本校內之網址）

1. 紙本重點整理講義

課程輔導時間

E-mail 預約。(原則以周一至周五、09:00-16:00)

聯合國全球永續發展目標(連結網址)

提供體驗課程：N

請尊重智慧財產權及性別平等意識，不得非法影印他人著作。
更新日期西元年/月/日：無	列印日期西元年/月/日：2025 / 5 / 09
MyTB教科書訂購平台：http://www.mytb.com.tw/