| Course Name |
(中) 農用分子生物技術與重組桿狀病毒表現系統(6937) |
| (Eng.) Technology of Agricultural Molecular Biology and Recombinant Baculovirus System |
| Offering Dept |
Master Program in Plant Health Care |
| Course Type |
Elective |
Credits |
3 |
Teacher |
TANG CHENG KANG |
| Department |
Doctoral Program in Plant Health Care / Ph.D |
Language |
Chinese |
英文/EMI |
|
Semester |
2025-SPRING |
| Course Description |
Baculovirus expression system is one of the most important technics that developed from insect science. It has not only been loaded in textbook, but also become a useful tool in biotechnological industry. For example, in pharmaceutical industry, there are some famous products produced by this technic, such as insulin, cytokines, growth factors, etc. Recombinant protein expression is the necessary technology behind this growing industry. This course will introduce the different expression systems used for recombinant protein production, the latest technologies of protein display and protein purification, and the fundamentals of fermentation. The Laboratory section will include the cloning of target genes into the expression vectors, protein purification, characterizations of the expressive products, and developments of their applications. |
| Prerequisites |
|
self-directed learning in the course |
N |
| Relevance of Course Objectives and Core Learning Outcomes(%) |
Teaching and Assessment Methods for Course Objectives |
| Course Objectives |
Competency Indicators |
Ratio(%) |
Teaching Methods |
Assessment Methods |
| Students are expected to learn the theory, practice, and the technology of agricultural molecular biology and recombinant baculovirus system, which would assist them to join the field of biopharmaceuticals in the future. |
|
|
| topic Discussion / Production |
| Exercises |
| Discussion |
| Practicum |
| Lecturing |
|
| Written Presentation |
| Attendance |
| Internship |
| Other |
|
| Course Content and Homework/Schedule/Tests Schedule |
| Week |
Course Content |
| Week 1 |
The course introduction and viewing the syllabus |
| Week 2 |
History of recombinant protein technologies |
| Week 3 |
Clinical applications: biopharmaceuticals and protein drugs |
| Week 4 |
Protein expression systems: Prokaryotic |
| Week 5 |
Protein expression systems: Eukaryotic (insect cells and mammalian cells) |
| Week 6 |
Genetic engineering of each expression system |
| Week 7 |
Protein surface display techniques |
| Week 8 |
Protein purification: Separation, precipitation, centrifugation, and filtration of cells and proteins |
| Week 9 |
Midterm report (期中考週) |
| Week 10 |
Chromatographic techniques for protein purification |
| Week 11 |
Design of a fermenter |
| Week 12 |
Biosensors and process control |
| Week 13 |
Cell line rearing: Sf9 and Sf21 |
| Week 14 |
Experiments: Cloning of the expression vectors for enzyme – 1 |
| Week 15 |
Experiments: Cloning of the expression vectors for enzyme - 2 |
| Week 16 |
Experiments: Recombinant protein purification |
| Week 17 |
Experiments: Enzymatic assay |
| Week 18 |
Final report (期末考週) |
|
| Evaluation |
課程分數與成績評量:
平時表現與出席: 40%
書面報告: 40%
實驗室實作: 20%
|
| Textbook & other References |
教科書:
1. Production of Recombinant Proteins: Novel Microbial and Eukaryotic Expression Systems (Editor: Gerd Gellissen) (ISBN: 978-3-527-31036-4, January 2005, Wiley-Blackwell, 429 Pages)
參考書目:
1. Cell Culture Engineering: Recombinant Protein Production (Advanced Biotechnology) (Editor: Gyun-Min Lee et al.) (ISBN-13: 978-3527343348, January 2020, Wiley-VCH, 440 Pages)
|
| Teaching Aids & Teacher's Website |
| 1. 紙本重點整理講義 |
| Office Hours |
| E-mail 預約。(原則以周一至周五、09:00-16:00) |
| Sustainable Development Goals, SDGs(Link URL) |
| | include experience courses：N |
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