| Relevance of Course Objectives and Core Learning Outcomes(%) |
Teaching and Assessment Methods for Course Objectives |
| Course Objectives |
Competency Indicators |
Ratio(%) |
Teaching Methods |
Assessment Methods |
培育具獨立思考、創新與研究能力之生醫專業人才
提供從事生物醫學領域研究的知識與技能
培養團隊合作精神與溝通協調整合能力
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| Written Presentation |
| Oral Presentation |
|
| Course Content and Homework/Schedule/Tests Schedule |
| Week |
Course Content |
| Week 1 |
1. Biochemistry of restriction enzymes |
| Week 2 |
2. Biochemistry of DNA modifying enzymes |
| Week 3 |
3. Cloning vectors: plasmids, phagemids, cosmids, PAC, BAC, YAC |
| Week 4 |
4. Competent cells and transformation |
| Week 5 |
5. DNA sequencing |
| Week 6 |
6. Southern blotting and Northern blotting analysis |
| Week 7 |
7. Semi-quantitative RT-PCR and quantitative real-time RT-PCR |
| Week 8 |
8. Determination of protein concentration |
| Week 9 |
9. Cell lysate preparation: whole cells or fractionation |
| Week 10 |
10. Electrophoresis for DNA and proteins |
| Week 11 |
11. Western blotting analysis |
| Week 12 |
12. Bioinformatic tools |
| Week 13 |
討論 |
| Week 14 |
討論 |
| Week 15 |
報告 |
| Week 16 |
報告
報告
測驗 |
self-directed
learning |
|
|
| Evaluation |
出席率(Attendance)：20%
課堂討論(Participation in Discussion)：40%
課後作業(Assignment)：40% |
| Textbook & other References |
| 自編教材 |
| Teaching Aids & Teacher's Website |
| 自編教材 |
| Office Hours |
| 星期三 15:00~17:00 |
| Sustainable Development Goals, SDGs(Link URL) |
| | include experience courses：N |
|