| 課程名稱 |
(中) 現代光譜學－生物物理學與生物化學解析(6889) |
| (Eng.) Modern Optical Spectroscopy: With Exercises And Examples From Biophysi |
| 開課單位 |
醫工所 |
| 課程類別 |
選修 |
學分 |
3 |
授課教師 |
林淑萍 |
| 選課單位 |
醫工所 / 碩士班 |
授課使用語言 |
英文 |
英文/EMI |
Y |
開課學期 |
1141 |
| 課程簡述 |
This course should give the knowledge of optical spectroscopy in solid-state molecules, cell biology, biophysics, and biochemistry. The course covers the introduction of optical instruments, atomic spectrum, spectra of proteins or nucleic acids, the photoelectric effect, fluorescence, IR and Raman spectroscopy, UV/VIS spectroscopy, Auger electron spectroscopy (AES), electron spectroscopy for chemical analysis (ESCA, also known as X-ray photoelectron spectroscopy (XPS)), Scanning Electron Microscopy (SEM) and EDS (energy-dispersive spectrometer) for the applications of chemical analysis, biochemistry, biophysics, and chemistry of surface material. |
| 先修課程名稱 |
|
課程含自主學習 |
Y |
| 課程與核心能力關聯配比(%) |
課程目標之教學方法與評量方法 |
| 課程目標 |
核心能力 |
配比(%) |
教學方法 |
評量方法 |
The main objective of this course is how to apply the concepts of optical spectroscopy to investigate and characterize the molecules, cell biology, biophysics, and biochemistry.
|
| 1.具備生醫工程領域之專業知識。 |
| 2.規劃並執行研究專題、具獨立解決問題之能力。 |
| 3.撰寫專業論文及表達研究成果之能力。 |
| 4.跨領域整合與創新研發之能力。 |
| 5.具良好的產業觀與國際觀。 |
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| 授課內容(單元名稱與內容、習作/每週授課、考試進度-共16週加自主學習) |
| 週次 |
授課內容 |
| 第1週 |
1. Introduction |
| 第2週 |
2. The Beer-Lambert Law
|
| 第3週 |
3. Absorption Spectra of Mixtures, Distortions of Absorption Spectra by Light Scattering or Nonuniform Distributions of the Absorbing Molecules |
| 第4週 |
4. UV/VIS spectra
|
| 第5週 |
5. Vibrational Adsorption
|
| 第6週 |
6. Absorption Spectra of Proteins and Nucleic Acids
|
| 第7週 |
7. IR spectra
|
| 第8週 |
8. Techniques for Measuring Absorbance
|
| 第9週 |
9. Introduction of the Photoelectric Effect
|
| 第10週 |
10.Lasers
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| 第11週 |
11.Raman spectra, ESCA spectra, and AES spectra
|
| 第12週 |
12.Analysis of ESCA spectra by XPS peak software
|
| 第13週 |
13.Fluorescence – (Fluorescence Yields and Lifetimes, Photobleaching, Fluorescent Probes and Tags)
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| 第14週 |
14.Single-Molecule, High-Resolution Microscopy, Scanning Electron Microscopy (SEM) and EDS (energy-dispersive spectrometer)
|
| 第15週 |
15.Final Exam
|
| 第16週 |
6.Project – Paper Reading and Discussion
Ϲ.Project – Paper Reading and Discussion
Ϻ.Final Report |
自主學習
內容 |
|
|
| 學習評量方式 |
Attendance 5%, Homework 15%, Midterm 25%, Final Exam 25%, Oral presentation 15%,Final Report 15%
|
| 教科書＆參考書目(書名、作者、書局、代理商、說明) |
1. Modern Optical Spectroscopy: With Exercises and Examples from Biophysics and Biochemistry, Springer, by William W. Parson, 2nd printing edition, 2009.2.
2. Photoelectron Spectroscopy: Principles and Applications, Springer Berlin Heidelberg, by Stephan Hüfner, 2010.
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| 課程教材（教師個人網址請列在本校內之網址） |
| iLearning |
| 課程輔導時間 |
splin@dragon.nchu.edu.tw
Wednesday, 1:00 pm-3:00 pm
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| 聯合國全球永續發展目標(連結網址) |
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