| 課程與核心能力關聯配比(%) |
課程目標之教學方法與評量方法 |
| 課程目標 |
核心能力 |
配比(%) |
教學方法 |
評量方法 |
| This is a one-semester course that serves as an introduction to the use of principles of insect tissue/cell culture. |
| 2.具備執行實驗、數據分析、歸納詮釋之能力 |
| 3.具備研究成果發表能力 |
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| 授課內容(單元名稱與內容、習作/每週授課、考試進度-共16週加自主學習) |
| 週次 |
授課內容 |
| 第1週 |
Introduction to insect tissue culture |
| 第2週 |
Aseptic concept and operating technique |
| 第3週 |
Experimental safety precautions, equipment introduction for insect tissue culture laboratory |
| 第4週 |
Basic aseptic operation and subculture |
| 第5週 |
The composition of the medium, the importance of each component and matters needing attention |
| 第6週 |
Preparation of medium, buffered saline and trypsin solution |
| 第7週 |
Cell count and viability analysis |
| 第8週 |
Mid-term exam |
| 第9週 |
Cell growth curve, cell storage and re-cultivation |
| 第10週 |
Cell subculture timing, subculture method and subculture practice |
| 第11週 |
Dyeing and photography |
| 第12週 |
Self-directed learning |
| 第13週 |
Self-directed learning |
| 第14週 |
Cell primary culture |
| 第15週 |
Concept and technology of cell selection and selective culture |
| 第16週 |
Single-cell colony formation assay
Baculovirus expression system
Final-term exam |
自主學習
內容 |
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| 學習評量方式 |
Mid-semester test results: 20%
Final test results: 20%
Written home assignments: 60% |
| 教科書＆參考書目(書名、作者、書局、代理商、說明) |
The course does not follow a particular textbook, material is assembled from various sources. Handouts will be distributed, books and articles treating special topics will be discussed and offered. Recommended major textbooks:
1. Culture of animal cells: a manual of basic technique 6th ed. R.I. Rewshney, 2010 Wiley-Liss published.
2. Insect cell culture: fundamental and applied aspects. J. M. Vlak, C. D. de Gooijer, J. Tramper, and H. G. Miltenburger, 2002 Kluwer academic Publishers. |
| 課程教材（教師個人網址請列在本校內之網址） |
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| 課程輔導時間 |
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| 聯合國全球永續發展目標(連結網址) |
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