NCHU Course Outline
Course Name (中) 生物化學實習(2254)
(Eng.) Biochemistry Laboratory
Offering Dept Department of Food Science and Biotechnology
Course Type Required Credits 2 Teacher TSAI,SHUO-WEN et al.
Department Department of Food Science and Biotechnology / Undergraduate Language Chinese Semester 2026-SPRING
Course Description 本課程主要分為四大部分,兼顧生物化學領域最重要的四個部份,由實驗中印證課本所學。分別由專精老師授課:1.共同基礎實驗課程。2.酵素動力學課程。3.核酸操作實驗課程。4.蛋白質純化課程。
Prerequisites
Relevance of Course Objectives and Core Learning Outcomes(%) Teaching and Assessment Methods for Course Objectives
Course Objectives Competency Indicators Ratio(%) Teaching Methods Assessment Methods
學生將生化課程最重要的四個部份,由實驗中印證課本所學,培養成為具有實作能力的優秀人才。及生化實驗之基本知識及操作技巧之傳授。
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2.
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60
20
20
Discussion
Practicum
Other
Lecturing
Assignment
Quiz
Written Presentation
Attendance
Internship
Other
Course Content and Homework/Schedule/Tests Schedule
Week Course Content
Week 1 課程解說、分組
Week 2 和平紀念日及進度調整停課
Week 3 胺基酸滴定時的緩衝現象
Week 4 蛋白質於不同 pH 環境之沉澱現象:等電點
Week 5 蛋白質定量
Week 6 旋光性質
Week 7 校慶及運動會補假
Week 8 還原糖定量:DNS法
Week 9 酵素反應趨勢
Week 10 酵素特性研究:最適溫度、最適pH
Week 11 酵素特性研究:熱安定性、pH耐受性
Week 12 微生物質體純化及DNA電泳
Week 13 質體的限制酶圖譜
Week 14 DNA片段放大技術:PCR
Week 15 蛋白質純化:色層分析1
Week 16 蛋白質純化:色層分析2 蛋白質電泳 操作考及期末筆試
self-directed
learning

Evaluation
書面報告,出席狀況,作業,測驗,實作等
上課反應、紙本考試、課堂的情境題及IRS按鈕練習、實際操作表現
Textbook & other References
自編教材、講義、IRS配合課程內容設計與之相符之題目上課即問即答、情境題教學、影片 (Diseases in digestive systems)、網上教材、短動畫
Teaching Aids & Teacher's Website
iLearning
Office Hours
每學期第一次上課時與選修學生商討後訂定
Sustainable Development Goals, SDGs(Link URL)
include experience courses:N
Please respect the intellectual property rights and use the materials legally.Please respect gender equality.
Update Date, year/month/day:None Printed Date, year/month/day:2026 / 4 / 16
The second-hand book website:http://www.myub.com.tw/