國立中興大學教學大綱

課程名稱	(中) 禽鳥胚胎實驗動物(6582)
	(Eng.) Avian Embryos as Model Animal
開課單位	生命碩士班
課程類別	選修	學分	2	授課教師	鄭旭辰
選課單位	生命碩士班 / 碩士班	授課使用語言	英文	英文/EMI	Y	開課學期	1141
課程簡述	介紹禽鳥胚胎發育的過程與特色，一般使用實驗動物的需求與條件，設計動物實驗須注意的事項，動物實驗結果蒐集與分析。
先修課程名稱						課程含自主學習	Y

課程與核心能力關聯配比(%)

課程目標之教學方法與評量方法

課程目標

核心能力

配比(%)

教學方法

評量方法

1. 培養學生能使用禽鳥胚胎作為實驗動物並獨立設計實驗的能力

4.(甲組、乙組)培養實驗分析寫作能力

6.(乙組)瞭解生物發生的現象及調控機制

8.(丙組)了解生物分子的功能和調節作用

講授

討論

口頭報告

作業

授課內容(單元名稱與內容、習作/每週授課、考試進度-共16週加自主學習)

週次	授課內容
第1週	Introduction to the avian embryos
第2週	Developmental features of avian embryo
第3週	General guidelines of designing and performing animal experiments
第4週	Collecting and analyzing data from animal experiments
第5週	The use of avian embryos in studying: (1) Angiogenesis (2) Metastasis
第6週	國慶連續假期停課一週
第7週	The use of avian embryos in studying: (3) Neurogenesis (4) Gonadonogenesis
第8週	The use of avian embryos in studying: (5) Cell migration (6) Toxicity
第9週	The use of avian embryos in studying: (7) Teratology (8) Stem cell biology
第10週	Spontaneous learning (自主學習)
第11週	Preparation of animal experiments Surgical procedures of animal experiment
第12週	Molecular tools for animal experiment: Transgenic
第13週	Molecular tools for animal experiment: Gene editing
第14週	Molecular tools for animal experiment: Viral vectors: Lentiviral vector, retroviral vector, Adenoviral vector
第15週	Molecular tools for animal experiment: RNAi and microRNA
第16週	Avian Genomics Challenges in avian animal model 期末討論
自主學習內容

學習評量方式

50 % Class discussion, 50% laboratory experiment

教科書＆參考書目(書名、作者、書局、代理商、說明)

10/7
Genomic Organization and Expression of the Doublesex-Related Gene Cluster in Vertebrates and Detection of Putative Regulatory Regions for DMRT1. GENOMICS Vol. 77, Numbers 1–2, September 2001
Expression profiling of purified mouse gonadal somatic cells during the critical time window of sex determination reveals novel candidate genes for human sexual dysgenesis syndromes. Human Molecular Genetics, 2006, Vol. 15, No. 3 417–431
Expression of Dmrt1 in the Genital Ridge of Mouse and Chicken Embryos Suggests a Role in Vertebrate Sexual Development. Developmental Biology 215, 208–220 (1999)

10/14
Cytochrome P450 aromatase expression in human seminoma
Reproductive Biology and Endocrinology 2005, 3:72
Effect of luteinising-hormone in vivo on ovarian cell subpopulations of newly-hatched chicks. British Poultry Sciencde (2000) 41: 494–501
Onset of meiosis in the chicken embryo; evidence of a role for retinoic acid. BMC Developmental Biology 2008, 8:85
Germ Cell-Intrinsic and -Extrinsic Factors Govern Meiotic Initiation in Mouse Embryos. Science 322, 1685-87 (2008);
Retinoic acid regulates sex-specific timing of meiotic initiation in mice. 2474–2479 _ PNAS _ February 21, 2006 _ vol. 103 _ no. 8
Approaches to define the role of SF-1 at different levels of the hypothalamic-pituitary-steroidogenic organ axis. Molecular and Cellular Endocrinology 179 (2001) 33–37

10/21
Proteome Analysis of Chicken Embryonic Gonads: Identification of Major Proteins From Cultured Gonadal Primordial Germ Cells. MOLECULAR REPRODUCTION AND DEVELOPMENT 72:521–529 (2005)
Using real time RT-PCR analysis to determine multiple gene expression patterns during XX and XY mouse fetal gonad development. Gene Expression Patterns 5 (2004) 141–149
Profiles of mRNA Expression of Genes Related to Sex Differentiation of the Gonads in the Chicken Embryo. 2003 Poultry Science 82:1462–1467
Sex Differentiation and mRNAExpression of P450c17, P450arom andAMH in Gonads
of the Chicken. MOLECULAR REPRODUCTION AND DEVELOPMENT 55:20–30 (2000)
Sexually dimorphic gene expression in the developing mouse gonad. Gene Expression Patterns 2 (2002) 359–367
The unique genomic properties of sex-biased genes: Insights from avian microarray data. BMC Genomics 2008, 9:148
Sexually dimorphic gene expression in the chick brain before gonadal differentiation. 2009 Poultry Science 88 :1003–1015

10/28
Differentiation of Female Primordial Germ Cells in the Male Testes of Chicken. MOLECULAR REPRODUCTION AND DEVELOPMENT 74:68–75 (2007)
Five Azacytidine, A DNA Methyltransferase Inhibitor, Specifically Inhibits Testicular Cord Formation and Sertoli Cell Differentiation In Vitro. MOLECULAR REPRODUCTION AND DEVELOPMENT
Embryonic Testis Cord Formation and Mesonephric Cell Migration Requires the Phosphotidylinositol 3-Kinase Signaling Pathway. BIOLOGY OF REPRODUCTION 67, 1927–1935 (2002)
The Partial Female to Male Sex Reversal in Wnt-4- Deficient Females Involves Induced Expression of Testosterone Biosynthetic Genes and Testosterone Production, and Depends on Androgen Action. Endocrinology 146(9):4016–4023

11/4
Cell proliferation is necessary for the determination of male fate in the gonad. Developmental Biology 258 (2003) 264–276
Effects of FGF9 on embryonic Sertoli cell proliferation and testicular cord formation in the mouse. Int. J. Dev. Biol. 48: 637-643 (2004)
Mesonephric FGF signaling is associated with the development of sexually indifferent gonadal primordium in chick embryos. Developmental Biology 280 (2005) 150–161
Stabilization of b-catenin in XY gonads causes male-to-female sex-reversal. Human Molecular Genetics, 2008, Vol. 17, No. 19 2949 – 2955
Genetic programs that regulate testicular and ovarian development. Molecular and Cellular Endocrinology 265–266 (2007) 3–9

11/11
Homograft Implants of Young Testes, Ovaries and Ovotestes in the Intact Female Chick. J. Embryol. exp. Morph., Vol. 11, Part 4, pp. 637-648, December 1963
Heterotopic Transplantation of Testes in Newly Hatched Chickens and Subsequent Production of Offspring via Intramagnal Insemination. BIOLOGY OF REPRODUCTION 76, 598–603 (2007)
Mechanisms of gonadal morphogenesis are not conserved between chick and mouse. Developmental Biology 302 (2007) 132–142
Specification of germ layer identity in the chick gastrula. BMC Developmental Biology 2007, 7:91

課程教材（教師個人網址請列在本校內之網址）

ilearning

課程輔導時間

By appointment

聯合國全球永續發展目標(連結網址)

01.消除貧窮 02.消除飢餓 03.健康與福祉 04.教育品質 15.陸地生態

提供體驗課程：Y

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更新日期西元年/月/日：無	列印日期西元年/月/日：2025 / 6 / 14
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