| 國立中興大學教學大綱 |
| 課程名稱 | (中) 散彈槍定序原理與實習(5147) | ||||||||
| (Eng.) Shutgun Sequencing:Principle and Experiment | |||||||||
| 開課單位 | 基資所 | ||||||||
| 課程類別 | 選修 | 學分 | 2 | 授課教師 | 陳盈璁 | ||||
| 選課單位 | 生科系 / 學士班 | 授課使用語言 | 英文 | 英文/EMI | Y | 開課學期 | 1132 | ||
| 課程簡述 | Shotgun Sequencing is a foundational technique in genomics research. It involves fragmenting the genome into smaller pieces for DNA sequencing, followed by assembling the sequenced fragments by overlapping reads to generate the complete genome sequence. This approach allows for rapid and efficient sequencing of entire genomes. Shotgun Sequencing is crucial for identifying genetic variations, studying genome structures, and discovering new genes. It forms the basis of modern precision medicine and finds extensive applications in the research of various organisms, including humans, animals, plants, and microorganisms. Content in brief: The course is conducted entirely in English (EMI). It covers the principles of various high-throughput genomic sequencing technologies, with a focus on Shotgun Sequencing strategies. Practical sessions include hands-on experience with Illumina short-read sequencing and Nanopore long-read sequencing. Genomic bioinformatics instruction covers Linux workstation usage, command-line interface operations, scripting languages (shell script/python), and progresses from basic to advanced genomic assembly analysis and visualization software package installation and usage. Bacterial Genome Project: The course revolves around a Bacterial Genome Project, allowing participants to engage in various aspects such as genome DNA purification, quality control, preparation of different sequencing libraries, hands-on high-throughput sequencing experiments, and learning sequential data processing, assembly, annotation, comparative genomics, database operations, and visualization analysis. Teaching Methods: The course employs a combination of classroom lectures (including multimedia presentations), laboratory sessions, and computer-based bioinformatics practical classes. The instructors, with over twenty years of experience in executing diverse genomics projects, emphasize strategic comparisons and considerations for different platforms within genomics projects. Practical problem-solving skills for various technical issues encountered in genomics projects are covered. Facility Visits: As a unique feature, the course includes planned visits to a core facility during the semester. Led by the course instructor, students will have the opportunity to visit high-throughput genomic facilities, including large Illumina sequencing platforms, traditional Sanger automated sequencing, or third-generation sequencing facilities. |
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| 先修課程名稱 | 課程含自主學習 | Y | |||||||
| 課程與核心能力關聯配比(%) | 課程目標之教學方法與評量方法 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 課程目標 | 核心能力 | 配比(%) | 教學方法 | 評量方法 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| By the end of this course, students will be able to: Understand the principles and applications of Shotgun Sequencing and its role in modern genomics research. Gain hands-on experience with high-throughput sequencing technologies, including Illumina and Nanopore platforms, and understand their comparative strengths and limitations. Execute a complete bacterial genome project, encompassing DNA extraction, sequencing library preparation, data assembly, annotation, and comparative analysis. Develop bioinformatics skills, including command-line operations, scripting, and the use of advanced genomic analysis and visualization tools. Critically evaluate sequencing strategies and troubleshoot technical challenges in genomics projects. Apply genomic research techniques to broader contexts, such as identifying genetic variations, studying microbial diversity, and exploring applications in medicine and biotechnology. |
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| 授課內容(單元名稱與內容、習作/每週授課、考試進度-共18週) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| 學習評量方式 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Participation, Discussion, Reports | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 教科書＆參考書目(書名、作者、書局、代理商、說明) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Papers, Lab protocols, URL to the materials will be provided | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 課程教材（教師個人網址請列在本校內之網址） | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 課程輔導時間 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Monday 9-12 AM Thursday 9-12AM, 14-16PM |
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| 更新日期 西元年/月/日：2025/01/09 14:29:32 | 列印日期 西元年/月/日：2025 / 3 / 13 |
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