NCHU Course Outline
Course Name (中) 基因表現系統(6623)
(Eng.) Gene Expression System
Offering Dept Graduate Institute of Molecular Biology
Course Type Elective Credits 2 Teacher LIU HUNG-JEN
Department Graduate Institute of Molecular Biology / Graduate Language Chinese 英文/EMI Semester 2025-FALL
Course Description 本課程在使學生深入了解常用之蛋白質表現系統包括大腸桿菌、酵母菌、baculovirus display及其它病毒應用於蛋白質表現等系統。並使學生能有效的活用這些知識及技術於各個不同的專業研究與疫苗與診斷試劑之研發。
Prerequisites
self-directed learning in the course Y
Relevance of Course Objectives and Core Learning Outcomes(%) Teaching and Assessment Methods for Course Objectives
Course Objectives Competency Indicators Ratio(%) Teaching Methods Assessment Methods
本課程將著重於原核基因及真核基因之表達,並介紹常用之蛋白質表現系統。
1.Competence in Molecular Biology
2.Competence in Biotechnology
3.Abilities in scientific argumentation and problem identification
4.Skills in organization, presentation and communication
30
30
20
20
Lecturing
Attendance
Quiz
Course Content and Homework/Schedule/Tests Schedule
Week Course Content
Week 1 課程介紹及基因重組及表現技術介紹
Week 2 基因重組及表現技術介紹
Week 3 應用於基因重組之酵素介紹
Week 4 應用於基因重組之酵素介紹
Week 5 應用於基因重組之酵素介紹
Week 6 基因重組技術及方法
Week 7 原核及真核之表現系統之介紹
Week 8 E. coli 表現系統
Week 9 E. coli 表現系統
Week 10 E. coli 表現系統
Week 11 yeast表現系統
Week 12 yeast表現系統
Week 13 yeast表現系統
Week 14 Baculovirus及其它病毒應用於蛋白質表現之系統
Week 15 Baculovirus及其它病毒應用於蛋白質表現之系統
Week 16 Baculovirus及其它病毒應用於蛋白質表現之系統
哺乳類細胞表現系統及
其它表現系統 哺乳類細胞表現系統及
其它表現系統
self-directed
learning

Evaluation
期中考試(45%)
期末考試(45%)
平常成績(10%)
Textbook & other References
講義
Teaching Aids & Teacher's Website
ilearning 3
Office Hours
每週課程結束後
Sustainable Development Goals, SDGs(Link URL)
03.Good Health and Well-Being   08.Decent Work and Economic Growth   09.Industry, Innovation and Infrastructureinclude experience courses:N
Please respect the intellectual property rights and use the materials legally.Please respect gender equality.
Update Date, year/month/day:None Printed Date, year/month/day:2025 / 7 / 02
The second-hand book website:http://www.myub.com.tw/