| Relevance of Course Objectives and Core Learning Outcomes(%) |
Teaching and Assessment Methods for Course Objectives |
| Course Objectives |
Competency Indicators |
Ratio(%) |
Teaching Methods |
Assessment Methods |
2)生物科技研發能力的養成、(6)基因暨生化工程師基礎學識的養成。
|
| 1.Familiar with biotechnology theoretical background, foundations, developments, and expansion. |
| 2.Performs technically skilled experiments and research methods. |
| 5.Capable of planning and designing laboratory experiments. |
| 6.Capable of analyzing data and experimental results. |
| 7.Capable of writing research reports. |
|
|
|
| Quiz |
| Assignment |
| Attendance |
|
| Course Content and Homework/Schedule/Tests Schedule |
| Week |
Course Content |
| Week 1 |
膠體製備與細菌培養方法 (呂維茗老師) |
| Week 2 |
基因剪接選殖 |
| Week 3 |
勝任細胞之製備與細菌轉型作用
|
| Week 4 |
自膠體回收DNA與DNA定量分析 |
| Week 5 |
重組質體之篩選與鑑定 |
| Week 6 |
植物RNA之萃取與電泳分析 (蔡慶修老師) |
| Week 7 |
反轉錄與即時定量聚合酶連鎖反應 |
| Week 8 |
體外轉錄反應 |
| Week 9 |
RNA膠體分析 |
| Week 10 |
北方轉漬雜交法 |
| Week 11 |
蛋白質處理 (童鈺堂老師) |
| Week 12 |
蛋白質定量 |
| Week 13 |
膠體電泳 |
| Week 14 |
西方墨點分析 |
| Week 15 |
酵素結合免疫吸附分析法Enzyme-linked immunosorbent assay (ELISA) |
| Week 16 |
自主學習 |
self-directed
learning |
01.Participation in professional forums, lectures, and corporate sharing sessions related to industry-government-academia-research exchange activities.
02.Viewing multimedia materials related to industry and academia.
03.Preparing presentations or reports related to industry and academia.
06.Participation in field trips and outdoor instructional activities at other NCHU campuses or branches, including experimental forests or test sites.
|
|
| Evaluation |
三位老師分別於課程結束當週進行小考，實驗操作亦分為三個階段分別評分，六個分數取平均值計算。
|
| Textbook & other References |
1. Molecular Cloning: A Laboratory Manual, Ed. III, J Sambrooke, EF Fritsch, T Maniatis, Cold Spring Harbor Laboratory, NY, 1989.
2. Current Protocols in Molecular Biology-
FM Ausubell, R Brent, RE Kingston, DD Moore, JG
Greene Publishing Associates and Wiley-Interscience, New York. 1997.
3. Current Protocols in Protein Science, JE Coligan, BM Dunn, HL Ploegh, DW Speicher,
New York: John Wiley and Sons, Inc., 1998
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| Teaching Aids & Teacher's Website |
|
| Office Hours |
每周一下午5 PM~7 PM
|
| Sustainable Development Goals, SDGs(Link URL) |
| include experience courses：N |
|