| 週次 |
授課內容 |
| 第1週 |
Introduction to molecular virology
What are viruses?
Differences among viruses infecting plants, animals, and microbes
Brief overview of the course
|
| 第2週 |
Structures and classifications of viruses
Functions of viral capsid proteins
Different structures of viruses
ICTV classification of viruses
|
| 第3週 |
Genome strategies of viruses
Genome structures of viruses
Expression of viral genes in mono- and poly-cistronic systems
Canonical and non-canonical translation
|
| 第4週 |
Analysis of viral genome diversity
Mutation, recombination, and re-assortment
Concept of quasi-species
Adaptation of viruses to different hosts
|
| 第5週 |
Introduction to MEGA & DnaSP software
MEGA: Molecular Evolutionary Genetics Analysis
DnaSP: DNA Sequence Polymorphism
Hands-on practices
|
| 第6週 |
How does a virus establish an infection cycle?
Entry into the first cell (uncoating + translation)
Establishment of replication
Cell-to-cell movement
|
| 第7週 |
Translation with unconventional strategies
Cap-independent translation
Translational recoding strategies
Subgenomic RNAs as translational tools
Polyprotein strategy and proteolytic processing
Host factor hijacking
Regulation of protein ratios
|
| 第8週 |
DNA virus replication mechanism
Geminiviridae, Caulimoviridae, Nanoviridae
Nuclear import of viral DNA, viral proteins (e.g., Rep) interacting host proteins
Rolling circle replication (RCR)
|
| 第9週 |
RNA virus replication mechanism
Formation of replication complexes on host membranes
Recruitment of host proteins (chaperones, translation factors)
Organelle targeting (ER, chloroplast, mitochondria)
|
| 第10週 |
Virus movement
Cell-to-cell movement via plasmodesmata
Requirement for movement proteins (MPs)
Long-distance movement through phloem
|
| 第11週 |
Plant antiviral defense mechanisms
RNA silencing (PTGS and TGS)
Small RNA pathways in plant biology
Molecular mechanisms of plant antiviral immunity
|
| 第12週 |
Viral suppressors of RNA silencing
VSRs
Viral counter-defense strategies
Arms race between plants and viruses
|
| 第13週 |
Research methodologies in plant virology
Infectious clone and replicon
Functional genomics tools
Cellular and subcellular localization
|
| 第14週 |
Applications of plant viruses in biotechnology
Virus-induced gene silencing (VIGS)
Virus-induced overexpression (VIOX)
Virus-induced genome editing (VIGE)
Plant-derived biopharmaceuticals (molecular farming)
|
| 第15週 |
Emerging plant viruses and future challenges
Rapid diagnostics
Broad-spectrum resistance
Plant viruses in sustainable agriculture
|
| 第16週 |
Final examination |
自主學習
內容 |
02.閱覽產業及學術相關多媒體資料
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