| 週次 |
授課內容 |
| 第1週 |
The introduction of hybridoma techniques |
| 第2週 |
Fusion and maintenance of hybridoma cells |
| 第3週 |
Techniques for the production of monoclonal antibodies |
| 第4週 |
Characterization of monoclonal antibodies |
| 第5週 |
Purification of monoclonal antibodies |
| 第6週 |
Practice of antigen preparation |
| 第7週 |
Practice of immunization |
| 第8週 |
Practice of myeloma cell culture |
| 第9週 |
Practice of fusion |
| 第10週 |
Discussion and oral presentation |
| 第11週 |
Practice of screening-ELISA |
| 第12週 |
Practice of screening-IFA |
| 第13週 |
Practice of cloning |
| 第14週 |
Practice of freezing hybridoma cells |
| 第15週 |
Practice of expansion of hybridoma cells |
| 第16週 |
Practice of isotyping the monoclonal antibody
Practice of purification of antibody
Final experiment report |
自主學習 內容 |
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