| 週次 |
授課內容 |
| 第1週 |
Course introduction |
| 第2週 |
Preparation of experimental proposal |
| 第3週 |
Preparation of experimental proposal |
| 第4週 |
Presentation of proposal |
| 第5週 |
Sample preparation for proteomic analysis I:
Protein extraction by homogenization |
| 第6週 |
Sample preparation for proteomic analysis II:
Protein TCA/acetone precipitation |
| 第7週 |
Sample preparation for proteomic analysis III:
Quantitation of proteins
|
| 第8週 |
Protein separation by one-dimensional gel electrophoresis:
Mini-SDS-PAGE and Coomassie blue staining |
| 第9週 |
Mid-term exam. week |
| 第10週 |
Protein separation by two-dimensional gel electrophoresis I: Isoelectric focusing |
| 第11週 |
Protein separation by two-dimensional gel electrophoresis II:
SDS-PAGE, staining and scanning of gels |
| 第12週 |
Sample preparation for mass spectrometry analysis I: Spot picking, in-gel digestion and spotting |
| 第13週 |
Protein identification by peptide mass fingerprinting and database searching |
| 第14週 |
Sample preparation for iTRAQ analysis |
| 第15週 |
iTRAQ analysis for proteomic quantitation |
| 第16週 |
Presentation and discussion of experimental results
Turn in final experimental report
Final exam. week |
自主學習
內容 |
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