國立中興大學教學大綱

課程名稱	(中) 植物基因轉殖特論(6073)
	(Eng.) Special Topics on Plant Gene Transformation
開課單位	基資所
課程類別	選修			學分	2	授課教師	陳玉婷
選課單位	基資所 / 碩士班			授課使用語言	中文	英文/EMI		開課學期	1141
課程簡述	Food is the most important thing for the people. How to improve the yield and quality of crops to provide them with sufficient food is the top priority of agricultural experts. However, with the rapid changes in the global environment, the traditional variety improvement and the improvement of farming techniques are facing bottlenecks. Plant gene transfer provides a breakthrough. In this course, the current principles and technologies related to plant-based transgenesis, including the construction of transgenic vectors and the molecular detection of transgenics, are properly discussed, so that students have a comprehensive understanding of which is helpful for future employment or research on related topics.
先修課程名稱								課程含自主學習	N

課程與核心能力關聯配比(%)			課程目標之教學方法與評量方法
課程目標	核心能力	配比(%)	教學方法	評量方法
1. This course appropriately discusses the current principles and technologies related to plant-based transgenesis, including the construction of transgenic vectors and the molecular detection of transgenics. So that students may have a comprehensive understanding of which will be helpful for future employment or research on related topics.	1.具備基因體學暨生物資訊學領域之專業知識 4.學習基因體研究技術	40 60	實習 講授 討論	測驗 口頭報告 實作
授課內容(單元名稱與內容、習作/每週授課、考試進度-共16週加自主學習)
週次 授課內容 第1週 Development and Prospect of Plant Gene Transplantation Technology 第2週 Plant tissue culture regeneration technology and gene transfer principle 第3週 Agrobacterium gene transfer technology 第4週 Gene gun gene transfer technology 第5週 Electroporation gene transfer technology 第6週 Using viral vectors to express foreign genes 第7週 Gene editing by CRISPR 第8週 Application of plant gene transfer in agriculture 第9週 The application of plant gene transfer in preservation and transportation 第10週 The application of plant gene transfer in the pharmaceutical industry 第11週 Application of plant gene transfer in genome research 第12週 Molecular Analysis of Transgenic Plants (I) - Extraction of Nucleic Acids and Proteins 第13週 Molecular Analysis of Transgenic Plants (II) – PCR and RT-PCR 第14週 Molecular analysis of transgenic plants (III) -Southern blot and Northern blot analyses 第15週 Molecular analysis of transgenic plants (IV) –Western blot and microarry analyses 第16週 Safety assessment and management of transgenic plants 自主學習 內容    02.閱覽產業及學術相關多媒體資料    03.製作專題報告
學習評量方式
Midterm exam 30% Internship 40% Oral Presentation 30%
教科書＆參考書目(書名、作者、書局、代理商、說明)
Gene Transfer to Plants. By Potrykus, I. and Spanmgenberg, G.. Springer Press (1995) Cell Culture and Regeneration of Plant Tissue. By Su. W.W. Marcel Dekker Press (2002) Plant tolerance to abiotic stresses in agriculture: role of genetic engineering. By Cherry, J. H., Locy, R.D. and Rychter, A. Kluwer Academic Press (2000)
課程教材（教師個人網址請列在本校內之網址）
iLearning
課程輔導時間
Monday 17:00-18:00
聯合國全球永續發展目標(連結網址)
提供體驗課程：N

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