| 國立中興大學教學大綱 |
| 課程名稱 | (中) 微生物遺傳學(4062) | ||||||||
| (Eng.) Microbial Genetics | |||||||||
| 開課單位 | 植病系 | ||||||||
| 課程類別 | 選修 | 學分 | 3 | 授課教師 | 鄧文玲 | ||||
| 選課單位 | 植病系 / 學士班 | 授課使用語言 | 英文 | 英文/EMI | N | 開課學期 | 1072 | ||
| 課程簡述 | The course is intended to (1) present the current state of molecular bacteriology and (2) illustrate how contemporary genetic technology is being used to understand molecular mechanisms that control environmental adaptation and behaviors in bacteria. | ||||||||
| 先修課程名稱 | 課程含自主學習 | N | |||||||
| 課程與核心能力關聯配比(%) | 課程目標之教學方法與評量方法 | ||||||||||||||
| 課程目標 | 核心能力 | 配比(%) | 教學方法 | 評量方法 | |||||||||||
| The following topics will be emphasized in the course: (1) Identifying fundamental questions that are addressed in bacterial biology. (2) Introducing model systems that are used to investigate these questions. (3) Available genetic technology that can be used to manipulate bacterial genes. (4) Emerging areas of bacterial genetics that are relevant to microbial life on earth. |
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| 授課內容(單元名稱與內容、習作/每週授課、考試進度-共18週) | |||||||||||||||
| 1. Introduction to microbial genetics and microbial genomics (6 weeks) (1) Transcription, translation, and gene regulation in prokaryotes Reading assignment 1: Winkler et al., 2002. Thiamine derivatives bind messenger RNAs directly to regulate bacterial gene expression. Nature 419:952-956. (2) Mechanisms of genetic variation (3) Repairing DNA breaks by homologous recombination non-homologous end-joining (NHEJ) Reading assignment 2: Chayot et al., 2010. An end-joining repair mechanism in Escherichia coli. PNAS 107:2141-2146. (4) Plasmids (5) Current development in microbial genomics: comparative and functional genomics 2. Bacterial communication and regulation of gene expression (3 weeks) (1) Bacteria sense environmental changes via two-component regulatory systems (2) Bacteria talk to each other via quorum sensing mechanisms (iBiology seminar by Binnie Bassler, http://www.ibiology.org/ibioseminars/microbiology/bonnie-bassler-part-1.html) Reading assignment 3: Schauder et al., 2001. The LuxS family of bacterial autoinducers: biosynthesis of a novel quorum-sensing signal molecule. Mol. Microbiol. 41:463-476. 3. Genomic plasticity: moveable genes and phase variation (1 week) Reading assignment 4: Boles, et al., 2004. Self-generated diversity produces “insurance effects” in biofilm communities. PNAS 101:16630-16635. 4. Cellular differentiation of Caulobacter: stalked cell and swarmer cell (1 week) Reading assignment 5: Holtzendorff et al., 2004. Oscillating global regulators control the genetic circuit driving a bacterial cell cycle. Science 304:983-987. 5. Bacterial clock: circadian rhythm of cyanobacterium (1 week) (iBiology seminar by Joseph Takahashi, http://www.ibiology.org/ibioseminars/genetics-gene-regulation/joe-takahashi-part-1a.html) Reading assignment 6: Nishiwaki et al., 2007. A sequential program of dual phosphorylation of KaiC as a basis for circadian rhythm in cyanobacteria. EMBO J. 26:4029-4037. 6. Bacterial development: sporulation of Bacillus (1 week) (iBiology seminar by Richard Losick, http://www.ibiology.org/ibioseminars/microbiology/richard-losick-part-1.html) Reading assignment 7: Camp, A. H. and Losick, R. 2009. A feeding tube model for activation of a cell-specific transcription factor during sporulation in Bacillus subtilis. Genes & Dev 23:1014-1024. 7. Genetics of bacterial virulence (1 week) (iBiology seminar by Stanley Falkow, http://www.ibiology. org/ibioseminars/microbiology/stanley-falkow-part-2.html) Reading assignment 8: Higashi et al., 2002. Biological activity of the Helicobacter pylori virulence factor CagA is determined by variation on the tyrosine phosphorylation sites. PNAS 99:14428-14433. 8. Current biotechnological applications of bacterial genetics (2 weeks) (1) Gateway cloning technology and bacteriophage genetics (2) CRISPR-Cas system and bacterial immunity (iBiology seminar by Jennifer Doudna, http://www.ibiology.org/ibiomagazine/jennifer-doudna-genome-engineering-with-crispr-cas9-birth-of-a-breakthrough-technology.html) |
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| 學習評量方式 | |||||||||||||||
| Homework (50%), term paper (20%), and participation of in-class discussion (30%) | |||||||||||||||
| 教科書＆參考書目(書名、作者、書局、代理商、說明) | |||||||||||||||
| 1. Snyder L., and Champness, W. 2007. Molecular Genetics of Bacteria, 3rd ed. ASM Press, Washington, D.C., USA. 735 pp. 2. Dale, J. W. and Park, S. F. 2004. Molecular Genetics of Bacteria, 4th ed. John Wiley and Sons Ltd., Chichester, England, UK. 310 pp. 3. Streips, U. N. and Yasbin, R. E. 2002. Modern Microbial Genetics, 2nd ed. Wiley-Liss Inc. New York, USA. 657 pp. 4. Assigned journal articles |
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| 課程教材（教師個人網址請列在本校內之網址） | |||||||||||||||
| e-campus | |||||||||||||||
| 課程輔導時間 | |||||||||||||||
| Tuesdays 3-5 pm | |||||||||||||||
| 聯合國全球永續發展目標(連結網址) | |||||||||||||||
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| 請尊重智慧財產權及性別平等意識，不得非法影印他人著作。 | |
| 更新日期 西元年/月/日：無 | 列印日期 西元年/月/日：2025 / 5 / 09 |
| MyTB教科書訂購平台：http://www.mytb.com.tw/ | |