NCHU Course Outline
Course Name (中) 植物組織培養及實驗(3281)
(Eng.) Plant Tissue Culture & Exercise
Offering Dept Department of Life Sciences
Course Type Elective Credits 3 Teacher LIN JENG SHANE
Department Department of Life Sciences / Undergraduate Language Chinese 英文/EMI Semester 2025-FALL
Course Description 本課程講授植物組織培養基本原理、與實際操作技術。包括根器官培養、癒合組織誘導、器官發生、生長點培養、試管內繁殖、單倍體培育、細胞懸浮培養等。
Prerequisites
self-directed learning in the course Y
Relevance of Course Objectives and Core Learning Outcomes(%) Teaching and Assessment Methods for Course Objectives
Course Objectives Competency Indicators Ratio(%) Teaching Methods Assessment Methods
習得植物組織培養基本原理與操作技術
2.The methods and techniques of experiments
5.The investigations of organismal mechanisms
50
50
Practicum
Lecturing
Written Presentation
Attendance
Assignment
Course Content and Homework/Schedule/Tests Schedule
Week Course Content
Week 1 簡介
Week 2 植物組織培養定義、內容
Week 3 植物組織培養機組成:成分、濃縮母液配製、濃度計算方法
Week 4 重要名詞介紹
Week 5 組織培養發展概況
Week 6 培養用外植體部位
Week 7 無菌培養之建立
Week 8 組織培養之應用-1
Week 9 組織培養之應用-2
Week 10 組織培養之原理-1
Week 11 組織培養之原理-2
Week 12 培養方法:含培養基之物理性質
Week 13 滅菌及無菌操作
Week 14 培養環境
Week 15 培養程序與操作要領
Week 16 試管苗健化、馴化與移植
自主學習週-1 (專題1:培植體建立,於第9-12週間進行)
自主學習週-2 (參與生科院或生科系舉辦專題講座任一場,並繳交心得)
self-directed
learning

Evaluation
1. 研究報告研讀、評量
2. 實驗報告評量
3. 實際操作評量
Textbook & other References
1. Plant cell, tissue and organ culture. Edited by O.L. Gamborg and G.C. phillips Springer
2. Plant cell culture. Edited by R.A. Dixon and R.A. Gonzates. IRL PRESS
Teaching Aids & Teacher's Website
印發自編教材講義
Office Hours
預約
Sustainable Development Goals, SDGs(Link URL)
include experience courses:N
Please respect the intellectual property rights and use the materials legally.Please respect gender equality.
Update Date, year/month/day:None Printed Date, year/month/day:2025 / 7 / 02
The second-hand book website:http://www.myub.com.tw/