NCHU Course Outline
Course Name (中) 食品微生物學實習(3268)
(Eng.) Food Microbiology Lab
Offering Dept Department of Food Science and Biotechnology
Course Type Elective Credits 1 Teacher WEN, HSIAO-WEI et al.
Department Department of Food Science and Biotechnology / Undergraduate Language Chinese Semester 2026-SPRING
Course Description 學習常見的食品微生物檢測方式,包含MPN、iMVIC、API20E、PCR以及ELISA等,並了解各項檢測方式的原理與應用。
Prerequisites 微生物學實習(一),微生物學實習(二),食品微生物學
Relevance of Course Objectives and Core Learning Outcomes(%) Teaching and Assessment Methods for Course Objectives
Course Objectives Competency Indicators Ratio(%) Teaching Methods Assessment Methods
讓學生學習以不同技術檢測出食品中病原菌
1.
2.
4.
30
40
30
Practicum
Attendance
Assignment
Internship
Quiz
Course Content and Homework/Schedule/Tests Schedule
Week Course Content
Week 1 課程介紹; 打掃環境;乾熱濕熱滅菌介紹
Week 2 (1) Sampling and sample handling
(2) Aerobic plate counts of foods
Week 3 (3) Direct microscopic counts of foods
Week 4 (4) Spiral plate method
(5) Psychrotrophs and thermodurics of milk
Week 5 (6) Mesophilic spores in foods
Week 6 (7) Bacteriological examination of food equipment and eating utensils
Week 7 (8) Enumeration of yeast and molds in foods
Week 8 (9) Fungi in foods
Week 9 期中考
Week 10 腸桿菌科檢測_鑑別試驗(公告方法)
Week 11 腸桿菌科檢測_生化試驗(公告方法)
Week 12 大腸桿菌檢測_鑑別試驗(公告方法)
Week 13 大腸桿菌檢測_生化試驗(公告方法)
Week 14 Rapid One for E. coli (快篩)
Week 15 PCR for Bacillus cereus (公告方法)
Week 16 qPCR for Salmonella sp. (公告方法) Lateral flow assay for Listeria sp. (快篩) 期末考試
self-directed
learning

Evaluation
期中、期末考50%
作業40%
出席狀況10%
Textbook & other References
1. Johnson, T.R., and C.L. Case. 2007. Laboratory experiments in microbiology. Pearson Education, Inc. San Francisco, CA. USA.
2. Post, F.J. 1998. Laboratory Manual for Food Microbiology and Biotechnology, 2nd ed. Star Publishing Company. Belmont, CA. USA.
3. FDA. Bacterial Analytical Manual Online [http://www.cfsan.fda.gov/~ebam/bam-toc.html]
4. 行政院衛生署。常用食品檢驗方法專輯 (一)~(三)。1991.
Teaching Aids & Teacher's Website
請見本課程iLearning
Office Hours
週二下午4-6點
Sustainable Development Goals, SDGs(Link URL)
 include experience courses:N
Please respect the intellectual property rights and use the materials legally.Please respect gender equality.
Update Date, year/month/day:None Printed Date, year/month/day:2026 / 4 / 30
The second-hand book website:http://www.myub.com.tw/