| 週次 |
授課內容 |
| 第1週 |
分組,上課須知,作業要求 |
| 第2週 |
Nematodes from the water environment |
| 第3週 |
General identification: head & esophagus regions, perineal pattern |
| 第4週 |
General identification: J2, male & female characters, perineal pattern |
| 第5週 |
General identification: above-ground nematodes; Inoculate RK nematode (I) |
| 第6週 |
Culturing nematodes: monoxenic, carrot disc and fungi hyphae slant culture (A) |
| 第7週 |
Spring break |
| 第8週 |
Submit the first field collecting samples |
| 第9週 |
Campus sampling sites |
| 第10週 |
Staining egg masses and counting galling index (II) |
| 第11週 |
Inoculate nematodes back to slant and tissue culture (B) |
| 第12週 |
Observing samples (DNA extraction) |
| 第13週 |
Extract DNA and select the PCR reactions |
| 第14週 |
PCR results and some questions—design primers for detection |
| 第15週 |
Oral presentation of the nematode identification from field samples特定作物的植物線蟲種類報告 |
| 第16週 |
Final exam (跑台)
|
自主學習 內容 |
   03.製作專題報告
|