NCHU Course Outline
Course Name (中) 微生物學操作技術(5163)
(Eng.) Experiments of Microbiology
Offering Dept Department of Life Sciences
Course Type Elective Credits 2 Teacher YU JU LIN
Department Department of Life Sciences / Graduate Language Chinese 英文/EMI Semester 2024-FALL
Course Description 這是藉由實驗操作來瞭解微生物的課程。課程中可以學習分離、培養微生物的技術,熟悉研究微生物基本的細胞、生理、生化等特性的方法,最後則為完成全套遺傳工程實驗。
Prerequisites
self-directed learning in the course N
Relevance of Course Objectives and Core Learning Outcomes(%) Teaching and Assessment Methods for Course Objectives
Course Objectives Competency Indicators Ratio(%) Teaching Methods Assessment Methods
本課程的目標是要讓學生熟悉研究微生物的一些基本但重要的方法與技術,使微生物學正課所學能在實驗操作中得到印證而加深瞭解,並有助於日後從事微生物學、生物化學、分子生物學等等相關領域的研究,或投身業界時所需。
7.Training the ability of performing biotechnological experiments
100
Practicum
Lecturing
Written Presentation
Attendance
Quiz
Internship
Course Content and Homework/Schedule/Tests Schedule
Week Course Content
Week 1 1. Introduction
Week 2 2. Techniques for isolation of pure culture
Week 3 3. Bacterial staining: Simple staining, negative staining, Gram stain
Week 4 4. Bacterial staining: Spore and capsule stain
Week 5 5. Nutritional requirements: Media for the routine cultivation of bacteria
Week 6 6. The bacterial growth curve
Week 7 7. Use of differential and selective media
Week 8 8. Extra-cellular enzymatic activities of microorganisms
Week 9 9. IMViC test
Week 10 10. Carbohydrate fermentation
Week 11 11. Cultivation and enumeration of bacteriophages
Week 12 12. Isolation of plasmid, bacteriophage, and chromosome DNA
Week 13 13. Recombinant DNA technique (I)
Week 14 14. Recombinant DNA technique (II)
Week 15 15. Cultivation and enumeration of bacteriophages
Week 16 16. 16S Library preparation
Week 17 17. Bioinformatic analysis
Week 18 18. Final exam
Evaluation
實驗報告與期中期末測驗為主,隨堂測驗與上課出席情形為輔。
Textbook & other References
無指定教科書(自編講義)
Teaching Aids & Teacher's Website
ilearning
Office Hours
洽詢授課老師
Sustainable Development Goals, SDGs
 include experience courses:N
Please respect the intellectual property rights and use the materials legally.Please repsect gender equality.
Update Date, year/month/day:None Printed Date, year/month/day:2025 / 1 / 03
The second-hand book website:http://www.myub.com.tw/