| 週次 |
授課內容 |
| 第1週 |
Introduction
|
| 第2週 |
Cell culture |
| 第3週 |
Freezing of cultured cells |
| 第4週 |
Propagation of viruses |
| 第5週 |
Titration of virus with TCID50 |
| 第6週 |
Preview |
| 第7週 |
Reverse transcription (RT)-PCR (1): Isolation of viral RNA from cells |
| 第8週 |
Reverse transcription (RT)-PCR (2): Reverse transcription of viral RNA
|
| 第9週 |
Reverse transcription (RT)-PCR (3): PCR using viral cDNA as a template
|
| 第10週 |
Reverse transcription (RT)-PCR (4): Agarose gel electrophoresis of PCR products |
| 第11週 |
Preview |
| 第12週 |
Plaque assay: Staining and counting plaques, determination of viral titers |
| 第13週 |
1. Extraction of cellular/viral DNA
2. Detection of viral DNA by PCR |
| 第14週 |
1. Nested PCR to detect small amounts of viral DNA
2. Viral growth curve determination (I) |
| 第15週 |
1. Viral growth curve determination (II)
2. Immunocytochemistry staining of virus in infected cells
|
| 第16週 |
Final Examination
製作實習成果報告 |
自主學習 內容 |
   03.製作專題報告
|