| Relevance of Course Objectives and Core Learning Outcomes(%) |
Teaching and Assessment Methods for Course Objectives |
| Course Objectives |
Competency Indicators |
Ratio(%) |
Teaching Methods |
Assessment Methods |
| 了解並實際操作病毒實驗基本步驟，以銜接高年級之副臨床及臨床課程。 |
|
|
| Practicum |
| Lecturing |
| Discussion |
|
| Written Presentation |
| Assignment |
| Internship |
| Attendance |
| Oral Presentation |
|
| Course Content and Homework/Schedule/Tests Schedule |
| Week |
Course Content |
| Week 1 |
Introduction
|
| Week 2 |
Cell culture |
| Week 3 |
Freezing of cultured cells |
| Week 4 |
Propagation of viruses |
| Week 5 |
Titration of virus with TCID50 |
| Week 6 |
Preview |
| Week 7 |
Reverse transcription (RT)-PCR (1): Isolation of viral RNA from cells |
| Week 8 |
Reverse transcription (RT)-PCR (2): Reverse transcription of viral RNA
|
| Week 9 |
Reverse transcription (RT)-PCR (3): PCR using viral cDNA as a template
|
| Week 10 |
Reverse transcription (RT)-PCR (4): Agarose gel electrophoresis of PCR products |
| Week 11 |
Preview |
| Week 12 |
Plaque assay: Staining and counting plaques, determination of viral titers |
| Week 13 |
1. Extraction of cellular/viral DNA
2. Detection of viral DNA by PCR |
| Week 14 |
1. Nested PCR to detect small amounts of viral DNA
2. Viral growth curve determination (I) |
| Week 15 |
1. Viral growth curve determination (II)
2. Immunocytochemistry staining of virus in infected cells
|
| Week 16 |
Final Examination
製作實習成果報告 |
self-directed
learning |
03.Preparing presentations or reports related to industry and academia.
|
|
| Evaluation |
1.繳交實習報告
2.學期測驗 |
| Textbook & other References |
1.F. J. Fenner. 1999. Veterinary Virology.
2.G.. C. Rovozzo. 1985. A Manual of Basic Virological Techniques.
|
| Teaching Aids & Teacher's Website |
| Students can access iLearning to get the handout. |
| Office Hours |
| Students need to make an appointment with teachers in advance. |